The Arabidopsis Nodulin Homeobox Factor AtNDX Interacts with AtRING1A/B and Negatively Regulates Abscisic Acid Signaling.,The Plant Cell

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The Arabidopsis Nodulin Homeobox Factor AtNDX Interacts with AtRING1A/B and Negatively Regulates Abscisic Acid Signaling.
The Plant Cell ( IF 10.0 ) Pub Date : 2020-01-09 , DOI: 10.1105/tpc.19.00604
Yujuan Zhu _{1,

2} , Xiaoying Hu ₁ , Ying Duan _{1,

3} , Shaofang Li ₁ , Yu Wang ₁ , Amin Ur Rehman ₁ , Junna He ₁ , Jing Zhang ₁ , Deping Hua ₁ , Li Yang ₁ , Li Wang ₁ , Zhizhong Chen ₁ , Chuanyou Li ₄ , Baoshan Wang ₅ , Chun-Peng Song ₆ , Qianwen Sun ₇ , Shuhua Yang ₁ , Zhizhong Gong ₈

Affiliation

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518100, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Key Lab of Plant Stress Research, College of Life Science, Shandong Normal University, Ji'nan, 250000, China.
Collaborative Innovation Center of Crop Stress Biology, Henan Province, Institute of Plant Stress Biology, Henan University, Kaifeng, 475001, China.
Center for Plant Biology and Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

The phytohormone abscisic acid (ABA) and the Polycomb group proteins have key roles in regulating plant growth and development; however, their interplay and underlying mechanisms are not fully understood. Here, we identified an Arabidopsis (Arabidopsis thaliana) nodulin homeobox (AtNDX) protein as a negative regulator in the ABA signaling pathway. AtNDX mutants are hypersensitive to ABA, as measured by inhibition of seed germination and root growth, and the expression of AtNDX is downregulated by ABA. AtNDX interacts with the Polycomb Repressive Complex1 (PRC1) core components AtRING1A and AtRING1B in vitro and in vivo, and together, they negatively regulate the expression levels of some ABA-responsive genes. We identified ABA-INSENSITIVE (ABI4) as a direct target of AtNDX. AtNDX directly binds the downstream region of ABI4 and deleting this region increases the ABA sensitivity of primary root growth. Furthermore, ABI4 mutations rescue the ABA-hypersensitive phenotypes of ndx mutants and ABI4-overexpressing plants are hypersensitive to ABA in primary root growth. Thus, our work reveals the critical functions of AtNDX and PRC1 in some ABA-mediated processes and their regulation of ABI4.

中文翻译：

拟南芥结节蛋白同源盒因子 AtNDX 与 AtRING1A/B 相互作用并负向调节脱落酸信号传导。

植物激素脱落酸（ABA）和多梳蛋白在调节植物生长和发育中发挥着关键作用；然而，它们的相互作用和潜在机制尚未完全了解。在这里，我们鉴定了拟南芥 (Arabidopsis thaliana) 结节蛋白同源盒 (AtNDX) 蛋白作为 ABA 信号通路中的负调节因子。 AtNDX 突变体对 ABA 高度敏感，通过抑制种子发芽和根生长来测量，并且 AtNDX 的表达被 ABA 下调。 AtNDX 在体外和体内与 Polycomb Repressive Complex1 (PRC1) 核心成分 AtRING1A 和 AtRING1B 相互作用，并共同负向调节一些 ABA 响应基因的表达水平。我们确定 ABA-INSENSITIVE (ABI4) 是 AtNDX 的直接目标。 AtNDX 直接结合 ABI4 的下游区域，删除该区域会增加初生根生长的 ABA 敏感性。此外，ABI4 突变挽救了 ndx 突变体的 ABA 过敏表型，并且 ABI4 过表达植物在初生根生长中对 ABA 过敏。因此，我们的工作揭示了 AtNDX 和 PRC1 在一些 ABA 介导的过程中的关键功能及其对 ABI4 的调节。

更新日期：2020-03-03

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