Colorimetric determination of the activity of alkaline phosphatase by exploiting the oxidase-like activity of palladium cube@CeO2 core-shell nanoparticles,Microchimica Acta

当前位置： X-MOL 学术 › Microchim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Colorimetric determination of the activity of alkaline phosphatase by exploiting the oxidase-like activity of palladium cube@CeO2 core-shell nanoparticles
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-01-09 , DOI: 10.1007/s00604-019-4070-9
Jiawei Wang ₁ , Pengjuan Ni ₁ , Chuanxia Chen ₁ , Yuanyuan Jiang ₁ , Chenghui Zhang ₁ , Bo Wang ₁ , Bingqiang Cao ₂ , Yizhong Lu ₁

Affiliation

Core-shell palladium cube@CeO2 (Pd cube@CeO2) nanoparticles are shown to display oxidase-like activity. This is exploited in a method for determination of the activity of alkaline phosphatase (ALP). The Pd cube@CeO2 nanoparticles were thermally synthesized from Ce(NO3)3, L-arginine and preformed Pd cube seeds in water. The Pd cube@CeO2 nanoparticles catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by oxygen. This results in the formation of oxidized TMB (oxTMB) with an absorption peak at 652 nm. Ascorbic acid (AA) is generated from the hydrolysis of L-ascorbic acid 2-phosphate (AAP) catalyzed by ALP. It can reduce oxTMB to TMB, and this results in a decrease of the absorbance. The method allows for quantitative determination of the activity of ALP in the range from 0.1 to 4.0 U·L−1 and with a detection limit down to 0.07 U·L−1. Endowed with high sensitivity and selectivity, the assay can quantify ALP activity in biological system with satisfactory results. Graphical abstract Schematic illustration of Pd cube@CeO2 core-shell nanoparticles for colorimetric determination of alkaline phosphatase. Schematic illustration of Pd cube@CeO2 core-shell nanoparticles for colorimetric determination of alkaline phosphatase.

中文翻译：

利用钯立方体@CeO2核壳纳米粒子的氧化酶样活性比色测定碱性磷酸酶的活性

核壳钯立方体@CeO2（Pd 立方体@CeO2）纳米颗粒显示出类似氧化酶的活性。这被用于测定碱性磷酸酶 (ALP) 活性的方法中。Pd 立方体@CeO2 纳米颗粒是由 Ce(NO3)3、L-精氨酸和预制的 Pd 立方体种子在水中热合成的。Pd立方体@CeO2纳米颗粒催化氧对3,3',5,5'-四甲基联苯胺（TMB）的氧化。这导致形成吸收峰为 652 nm 的氧化 TMB (oxTMB)。抗坏血酸 (AA) 是由 ALP 催化的 L-抗坏血酸 2-磷酸 (AAP) 水解产生的。它可以将 oxTMB 还原为 TMB，从而导致吸光度降低。该方法允许在 0.1 到 4.0 U·L-1 范围内定量测定 ALP 的活性，检测限低至 0.07 U·L-1。该方法具有较高的灵敏度和选择性，可以对生物系统中的ALP活性进行定量，结果令人满意。用于比色测定碱性磷酸酶的 Pd 立方体@CeO2 核壳纳米粒子的图形摘要示意图。Pd立方体@CeO2核壳纳米颗粒用于碱性磷酸酶比色测定的示意图。

更新日期：2020-01-09

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11