Abstract Based on the oxidation-redox mechanism of naturally occurring NADH along with its metal coordination properties, biogenic AuPd nanoclusters are directly synthesized with low concentration of metal precursors, showing outstanding peroxidase mimicking activities. Ultrasmall bimetallic nanozymes can rapidly form under the reduction and the stabilization of NADH, and their physicochemical properties are closely associated to the molar ratio of [Na2PdCl4]/[HAuCl4]. Synergistic effects between Au and Pd play a particular role in both growth kinetics and catalytic performance of AuPd nanoclusters. The strong Au-Pd interactions remarkably adjust the electronic properties of both Au and Pd species in bimetallic NCs, thereby contributing to their superior enzymatic activities over two monometallic counterparts. Based on the peroxidase-like activity of AuPd nanoclusters at acidic pH, a colorimetric test is developed for monitoring acid phosphatase quantitatively, giving the limit of detection of 0.53 U/L. The linear response lies in the concentration range of 1–14 U/L. This method is also suitable for estimating ACP activity in biological fluid.

中文翻译：

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AuPd纳米团簇作为过氧化物酶模拟物的生物合成及其在酸性磷酸酶比色分析中的应用

摘要 基于天然存在的 NADH 的氧化-氧化还原机制及其金属配位特性，生物源 AuPd 纳米团簇是用低浓度的金属前体直接合成的，具有出色的过氧化物酶模拟活性。超小双金属纳米酶在NADH的还原和稳定作用下可以快速形成，其理化性质与[Na2PdCl4]/[HAuCl4]的摩尔比密切相关。Au和Pd之间的协同效应在AuPd纳米团簇的生长动力学和催化性能中发挥着特殊作用。强烈的 Au-Pd 相互作用显着调整了双金属 NC 中 Au 和 Pd 物种的电子特性，从而有助于它们比两种单金属对应物优越的酶活性。基于AuPd纳米团簇在酸性pH下的类过氧化物酶活性，开发了一种用于定量监测酸性磷酸酶的比色测试，其检测限为0.53 U/L。线性响应位于 1–14 U/L 的浓度范围内。该方法也适用于估计生物体液中的 ACP 活性。