Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.,Cell

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Modulation of RNA Condensation by the DEAD-Box Protein eIF4A.
Cell ( IF 45.5 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.cell.2019.12.031
Devin Tauber ₁ , Gabriel Tauber ₁ , Anthony Khong ₂ , Briana Van Treeck ₁ , Jerry Pelletier ₃ , Roy Parker ₂

Affiliation

Stress granules are condensates of non-translating mRNAs and proteins involved in the stress response and neurodegenerative diseases. Stress granules form in part through intermolecular RNA-RNA interactions, and to better understand how RNA-based condensation occurs, we demonstrate that RNA is effectively recruited to the surfaces of RNA or RNP condensates in vitro. We demonstrate that, through ATP-dependent RNA binding, the DEAD-box protein eIF4A reduces RNA condensation in vitro and limits stress granule formation in cells. This defines a function for eIF4A to limit intermolecular RNA-RNA interactions in cells. These results establish an important role for eIF4A, and potentially other DEAD-box proteins, as ATP-dependent RNA chaperones that limit the condensation of RNA, analogous to the function of proteins like HSP70 in combatting protein aggregates.

中文翻译：

DEAD-Box 蛋白 eIF4A 对 RNA 缩合的调节。

应激颗粒是参与应激反应和神经退行性疾病的非翻译 mRNA 和蛋白质的凝聚物。应力颗粒部分通过分子间 RNA-RNA 相互作用形成，为了更好地了解基于 RNA 的凝聚是如何发生的，我们证明 RNA 在体外被有效地募集到 RNA 或 RNP 凝聚物的表面。我们证明，通过 ATP 依赖性 RNA 结合，DEAD-box 蛋白 eIF4A 在体外减少 RNA 凝聚并限制细胞中应力颗粒的形成。这定义了 eIF4A 限制细胞中分子间 RNA-RNA 相互作用的功能。这些结果确立了 eIF4A 和其他潜在的 DEAD-box 蛋白的重要作用，作为限制 RNA 凝聚的 ATP 依赖性 RNA 伴侣，

更新日期：2020-01-10

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