The increasing availability and affordability of natural gas has renewed interest in using methanol for bioproduction of useful chemicals. Engineering synthetic methylotrophy based on natural or artificial methanol assimilation pathways and genetically tractable platform microorganisms for methanol-based biomanufacturing is drawing particular attention. Recently, intensive efforts have been devoted to demonstrating the feasibility and improving the efficiency of synthetic methylotrophy. Various fuel, bulk, and fine chemicals have been synthesized using methanol as a feedstock. However, fully synthetic methylotrophs utilizing methanol as the sole carbon source and commercially viable bioproduction from methanol remain to be developed. Here, we review ongoing efforts to identify limiting factors, optimize synthetic methylotrophs, and implement methanol-based biomanufacturing. Future challenges and prospects are also discussed.

中文翻译：

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合成甲基营养：基于甲醇的生物制造的实用解决方案。

天然气日益增加的可用性和可承受性已经引起人们对使用甲醇进行有用化学品的生物生产的兴趣。基于天然或人工甲醇同化途径和基于遗传的可操作平台微生物进行基于甲醇的生物制造的工程合成甲基营养素正受到特别关注。近来，已经投入大量努力来证明可行性和提高合成甲基营养的效率。使用甲醇作为原料已经合成了各种燃料，散装和精细化学品。然而，利用甲醇作为唯一碳源的完全合成的甲基营养体和由甲醇在商业上可行的生物生产仍有待开发。在这里，我们回顾了为确定限制因素，优化合成甲基营养素，并实施基于甲醇的生物制造。还讨论了未来的挑战和前景。