Nitrogen cycling during composting process is not yet fully understood. This study explored the key genes involved in nitrogen cycling during dairy manure composting process using high-throughput sequencing and quantitative PCR technologies. Results showed that nitrogen fixation occurred mainly during the thermophilic and cooling phases, and significantly enhanced the nitrogen content of compost. was the main diazotroph. Ammonia oxidation occurred during the maturation phase and sp. was the most abundant ammonia oxidizing bacteria. Denitrification contributed to the greatest nitrogen loss during the composting process. The community was dominated by sp. and sp., while the community was dominated by and . The community varied in a succession of , and dominated communities according to different composting phases. Based on these results, nitrogen cycling models for different phases of the dairy manure composting process were established.

中文翻译：

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通过结合高通量测序和定量 PCR 深入了解奶牛粪便堆肥过程中氮循环的微生物学


堆肥过程中的氮循环尚未完全了解。本研究利用高通量测序和定量PCR技术探讨了奶牛粪便堆肥过程中参与氮循环的关键基因。结果表明，固氮作用主要发生在高温和冷却阶段，显着提高了堆肥的氮含量。是主要的固氮生物。氨氧化发生在成熟阶段和 sp。是最丰富的氨氧化细菌。反硝化作用在堆肥过程中造成最大的氮损失。该社区以 sp 为主。和 sp.，而社区则以 和 为主。根据不同的堆肥阶段，群落有一系列变化，并主导群落。基于这些结果，建立了奶牛粪便堆肥过程不同阶段的氮循环模型。