Sensitive and selective detection of microRNA in complex biological samples based on protein-enhanced fluorescence anisotropy,Analytical Methods

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Sensitive and selective detection of microRNA in complex biological samples based on protein-enhanced fluorescence anisotropy
Analytical Methods ( IF 2.7 ) Pub Date : 2020/01/10 , DOI: 10.1039/c9ay02024a
Yan Li _{1,

2} , Yao Sun _{1,

2} , Jieyun Ye _{2,

3} , Fengping Pan _{1,

2} , Bo Peng _{1,

2} , Haiqiang Li _{1,

2} , Mingming Zhang _{1,

2} , Yan Xu _{1,

2}

Affiliation

Here we report a molecular mass amplifying strategy to construct a fluorescence anisotropy sensor for the detection of microRNA. Because fluorescence anisotropy is less affected by biological matrix interferences, the proposed method can be used directly for the detection of targets in tumor cell lysates without any tedious sample pretreatment, and a good linearity in the 10 pM to 0.5 nM range with a detection limit down to 3.4 pM was achieved. The results established that our method can be used to design fluorescence anisotropy sensors for rapid, sensitive, and selective detection of miRNAs for early diagnosis of cancer.

中文翻译：

基于蛋白质增强的荧光各向异性的灵敏和选择性检测复杂生物样品中的微小RNA

在这里，我们报告一种分子质量扩增策略，以构建用于检测microRNA的荧光各向异性传感器。由于荧光各向异性受生物基质干扰的影响较小，因此该方法无需任何繁琐的样品预处理即可直接用于检测肿瘤细胞裂解物中的靶标，并且在10 pM至0.5 nM范围内具有良好的线性，且检测限降低达到3.4 pM。结果表明，我们的方法可用于设计荧光各向异性传感器，以快速，灵敏和选择性地检测miRNA，以早期诊断癌症。

更新日期：2020-02-13

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