A systematic study of the secondary metabolites of the wild granaticin-producing strain Streptomyces vietnamensis GIMV4.0001 led to the isolation of six known early shunt products related to actinorhodin, SEK34 (3), SEK34b (4), mutactin (5), dehydromutactin (7), EM18 (8) and GTRI-02 (9). While the other shunt products were minor or trace products, the production ratio of SEK34 (3) and SEK34b (4) to granaticins was strikingly high. Nearly 64% of the intermediate with the first ring closed went to the SEK34/SEK34b aberrant pathway. The high level of the aberrant metabolic flow toward the early shunt products SEK34 and SEK34b indicated that the second ring closure of the granaticin (1) biosynthesis is a key limiting step in the granaticin biosynthetic machinery of S. vietnamensis GIMV4.0001.

中文翻译：

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越南链霉菌GIMV4.0001的花青素生物合成机制中的早期分流产物出现异常的代谢流。

对野生产格拉那霉素的越南链霉菌GIMV4.0001的次生代谢产物的系统研究导致分离出六种与肌动蛋白，SEK34（3），SEK34b（4），mutactin（5），dehydromutactin（ 7），EM18（8）和GTRI-02（9）。尽管其他分流产品是次要产品或痕量产品，但SEK34（3）和SEK34b（4）与格拉金霉素的生产比例非常高。第一环闭合时，近64％的中间体进入SEK34 / SEK34b异常途径。朝向早期分流产物SEK34和SEK34b的异常代谢高水平流动表明，Granatinin（1）生物合成的第二个环闭合是越南葡萄球菌GIMV4.0001的Granaticin生物合成机制中的关键限制步骤。