BACKGROUND Several Tritrichomonas species have been found in mammalian hosts. Among these trichomonads T. foetus is often found in the urogenital tract of cattle and the gastrointestinal tract of the domestic cat, resulting in sexually transmitted bovine trichomonosis and fecal-orally transmitted feline trichomonosis, respectively. The aims of the current study were to molecularly characterize clinical isolates of T. foetus in cattle populations in Wyoming, South Dakota, and Montana of the United States of America and to phylogenetically analyze Tritrichomonas species of mammalian hosts. RESULTS DNA sequencing of rRNA genes showed over 99% identity of the newly described isolates to other bovine isolates. Further, T. foetus isolates of various mammalian hosts originated in different geographic regions worldwide were clustered into two well-defined clades by phylogenetic analysis of rRNA and cysteine protease 2 genes. Clade I consisted of isolates originated from cattle, pig, and human whereas clade II contained isolates of cat and dog. CONCLUSION It is concluded that all mammalian Tritrichomonas spp. apparently belong to T. foetus. Analysis of more sequences is warranted to support this conclusion.

中文翻译：

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美国怀俄明州、南达科他州和蒙大拿州公牛胎儿三滴虫的临床分离株。


背景技术已在哺乳动物宿主中发现了几种三滴虫属物种。在这些滴虫中，胎儿毛滴虫经常出现在牛的泌尿生殖道和家猫的胃肠道中，分别导致性传播的牛滴虫病和粪口传播的猫滴虫病。本研究的目的是对美国怀俄明州、南达科他州和蒙大拿州牛群中胎儿毛毛虫的临床分离株进行分子特征分析，并对哺乳动物宿主的毛滴虫属物种进行系统发育分析。结果 rRNA 基因的 DNA 测序显示，新描述的分离株与其他牛分离株的同一性超过 99%。此外，通过 rRNA 和半胱氨酸蛋白酶 2 基因的系统发育分析，来自世界各地不同地理区域的各种哺乳动物宿主的胎儿 T. fetus 分离物被聚类为两个明确的进化枝。进化枝 I 由源自牛、猪和人类的分离株组成，而进化枝 II 包含猫和狗的分离株。结论 结论是所有哺乳动物三滴虫属。显然属于T. foetus。有必要对更多序列进行分析来支持这一结论。