Ultrasound can be focused into deep tissues with millimeter precision to perform noninvasive ablative therapy for diseases such as cancer. In most cases, this ablation uses high intensity ultrasound to deposit nonselective thermal or mechanical energy at the ultrasound focus, damaging both healthy bystander tissue and cancer cells. Here, we describe an alternative low intensity (ISPTA 20 ms causes selective disruption of a panel of breast, colon, and leukemia cancer cell models in suspension without significantly damaging healthy immune or red blood cells. Mechanistic experiments reveal that the formation of acoustic standing waves and the emergence of cell-seeded cavitation lead to cytoskeletal disruption, expression of apoptotic markers, and cell death. The inherent selectivity of this low intensity pulsed ultrasound approach offers a potentially safer and thus more broadly applicable alternative to nonselective high intensity ultrasound ablation.

中文翻译：

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用低强度脉冲超声选择性消融癌细胞

超声波可以以毫米级精度聚焦到深层组织，对癌症等疾病进行无创消融治疗。在大多数情况下，这种消融使用高强度超声在超声焦点处沉积非选择性热能或机械能，从而损害健康的旁观者组织和癌细胞。在这里，我们描述了另一种低强度（ISPTA 20 ms 导致选择性破坏一组悬浮的乳腺癌、结肠癌和白血病癌细胞模型，而不会显着损害健康的免疫细胞或红细胞。机械实验表明声驻波的形成细胞接种空化的出现导致细胞骨架破坏、凋亡标志物表达和细胞死亡。