Neuroblastoma (NB) is the most common solid extracranial malignancy in children with a considerable chance of metastatic progression. Prevalent evidence supports the anti-tumor role of γδT cells and these cells have been testing in clinical trials for constraining tumor growth. A small subpopulation of γδT cells releasing IL-17, however, were demonstrated to exert tumor-promoting effects in many aspects. In this study, we found an augment of IL-17+ γδT cells both in in vitro PAM-stimulated γδT-cell expanding culture and circulating γδT cells in NB patients. These patient-origin cells expanded in vitro by PAM in the presence of IL-17 polarizing condition were shown to promote the proliferation and migration of NB cells. Furthermore, an intrinsic preference for IL-17 polarization in NB γδT cells was revealed by mRNA microarray and Western Blot, which pointed to an up-regulated expression of multiple Th17-development related genes in addition to an increased phosphorylation level of STAT3.

中文翻译：

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增加的产生IL-17的γδT细胞促进神经母细胞瘤中肿瘤细胞的增殖和迁移。

神经母细胞瘤（NB）是儿童中最常见的实体颅外恶性肿瘤，有相当大的转移进展机会。大量证据支持γδT细胞的抗肿瘤作用，并且这些细胞已经在临床试验中用于限制肿瘤生长。然而，一小部分释放IL-17的γδT细胞在许多方面都表现出促肿瘤作用。在这项研究中，我们发现NB患者在体外PAM刺激的γδT细胞扩展培养和循环的γδT细胞中均增加了IL-17 +γδT细胞。这些患者来源的细胞在存在IL-17极化条件下通过PAM在体外扩增，显示可促进NB细胞的增殖和迁移。此外，mRNA芯片和Western Blot揭示了NBγδT细胞中IL-17极化的内在偏好。