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Bioconjugation of Aptamer to Fluorescent Trimethyl Chitosan Nanoparticles for Bacterial Detection
Materials Letters ( IF 3 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.matlet.2020.127330
Xueqin Zhao , Chao Lu , Songlin Yang , Jin Zhang

Abstract Trimethyl chitosan nanoparticles (TMC NPs), the positively charged chitosan derivative, have been used to deliver medicines to overcome the tissue barrier. Here, fluorescein-labeled TMC NPs were produced by a microemulsion method following the bioconjugation of the single-strand DNA aptamer which can specifically bind to E. coli (DH5α). TMC NPs show uniform spherical particles with an average diameter of 323 ± 10 nm. The large positive zeta potential, 37.1 ± 2.2 mV, indicates the net positive charged surface of TMC NPs which is a suitable carrier for crossing tissue barriers. The linear relationship of the maximum fluorescence intensity of aptamer modified TMC NPs and the concentration of E. coli (DH5α) is obtained. We hope that the fluorescent TMC NPs integrating with a suitable aptamer could be used to understand the pathway of bacteria crossing the host barrier to fight against invasive infections.

中文翻译:

适体与荧光三甲基壳聚糖纳米颗粒的生物偶联用于细菌检测

摘要 三甲基壳聚糖纳米颗粒 (TMC NPs) 是一种带正电荷的壳聚糖衍生物,已被用于递送药物以克服组织屏障。在这里,荧光素标记的 TMC NPs 是在单链 DNA 适体的生物偶联后通过微乳液法生产的,该适体可以与大肠杆菌 (DH5α) 特异性结合。TMC NPs 显示出均匀的球形颗粒,平均直径为 323 ± 10 nm。37.1 ± 2.2 mV 的大正 zeta 电位表明 TMC NPs 的净正电荷表面是穿过组织屏障的合适载体。获得了适体修饰的 TMC NPs 的最大荧光强度与大肠杆菌 (DH5α) 浓度的线性关系。
更新日期:2020-04-01
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