Mycotoxin contamination in food and feed is a global concern because mycotoxin contamination can cause both acute and chronic health effects in humans and animals. In the present work, an Escherichia coli-based biosensor is described for the toxicity assessment of aflatoxin B1 (AFB1) and zearalenone (ZEN). In this electrochemical biosensor, E. coli is used as the signal recognition element, p-benzoquinone is used as the mediator, and a two-step reaction procedure has been developed to separate the mediator from the mycotoxins. The current value of the as-prepared microbial biosensor exhibits a linear decrease with concentrations of AFB1 and ZEN in the range of 0.01-0.3 and 0.05-0.5 μg/mL, with detection limits reaching 1 and 6 ng/mL, respectively. The IC25 values obtained by the present method are 0.25 and 0.40 μg/mL for AFB1 and ZEN, which shows that the cytotoxicity of AFB1 to E. coli is more severe than the cytotoxicity of ZEN to E. coli. The combined toxic effect of these two mycotoxins has also been explored, and synergistic biotoxicity has been observed. Moreover, the biosensor is successfully applied to the toxicity evaluation of mycotoxins in real samples, including peanut and corn oils. This work could provide new insight into mycotoxin and microorganism interactions and could establish a new approach for future mycotoxin detection.

中文翻译：

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用于真菌毒素毒性检测的基于大肠杆菌的电化学生物传感器的开发。

食品和饲料中的霉菌毒素污染已成为全球关注的问题，因为霉菌毒素污染可对人类和动物造成急性和慢性健康影响。在当前的工作中，描述了一种基于大肠杆菌的生物传感器，用于黄曲霉毒素B1（AFB1）和玉米赤霉烯酮（ZEN）的毒性评估。在该电化学生物传感器中，大肠杆菌被用作信号识别元件，对苯醌被用作介体，并且已经开发出两步反应程序以将介体与霉菌毒素分离。所制备的微生物生物传感器的当前值随着AFB1和ZEN的浓度在0.01-0.3和0.05-0.5μg/ mL的范围内呈现线性下降，检测极限分别达到1和6 ng / mL。对于AFB1和ZEN，通过本方法获得的IC25值为0.25和0.40μg/ mL，这表明AFB1对大肠杆菌的细胞毒性比ZEN对大肠杆菌的细胞毒性更严重。还研究了这两种霉菌毒素的联合毒性作用，并观察到了协同的生物毒性。此外，该生物传感器已成功应用于霉菌毒素在真实样品（包括花生油和玉米油）中的毒性评估。这项工作可以提供有关霉菌毒素和微生物相互作用的新见解，并可以为将来的霉菌毒素检测建立新的方法。包括花生油和玉米油。这项工作可以提供有关霉菌毒素和微生物相互作用的新见解，并可以为将来的霉菌毒素检测建立新的方法。包括花生油和玉米油。这项工作可以提供有关真菌毒素和微生物相互作用的新见解，并可以为将来检测真菌毒素建立新的方法。