Amylosucrase (AS; EC 2.4.1.4) is an enzyme that has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize α-1,4-glucans, like amylose, from sucrose as a sole substrate, but importantly, it can also utilize various other molecules as acceptors. In addition, AS produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. Furthermore, AS produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by AS forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences. This review not only compares the gene and enzyme characteristics of microbial AS, studied to date, but also focuses on the applications of AS in the biotechnology and food industries.

中文翻译：

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淀粉蔗糖酶（一种新型葡萄糖基转移酶）的多功能生物技术应用。

淀粉蔗糖酶（AS；EC 2.4.1.4）是一种由于其多功能酶活性而在生物技术和食品工业中具有巨大潜力的酶。它可以以蔗糖为唯一底物合成α-1,4-葡聚糖，如直链淀粉，但重要的是，它还可以利用各种其他分子作为受体。此外，AS还产生蔗糖异构体，例如松二糖和海藻酮糖。它还可以有效合成增加慢消化淀粉和抗性淀粉比例的变性淀粉，以及增加水溶性和稳定性的糖基化功能化合物。此外，AS 比其他碳水化合物活性酶更有效地产生转角糖。AS 合成的直链淀粉形成微粒，这些微粒可用作具有各种生物应用的生物相容性材料，包括药物输送、色谱、和生物分析科学。本综述不仅比较了迄今为止研究的微生物AS的基因和酶特征，还重点讨论了AS在生物技术和食品工业中的应用。