Abstract

The in vivo decomposition of regenerated cellulose in the digestive organs of a mouse was investigated for the first time, in anticipation of food products of regenerated cellulose becoming commercially available. In past studies, plants were grown from their seeds in a labeled CO₂ environment and labeled cellulose was then extracted from these plants. The small amounts of the obtained labeled cellulose made the study of in vivo decomposition of cellulose in a digestive tract difficult. In the present study, a new method was developed to overcome this. ¹³C-labeled natural cellulose (¹³C-NC) was produced from cultures of Gluconacetobacter xylinus supplemented with ¹³C-labeled glucose. ¹³C-labeled regenerated cellulose was then prepared from a hydrolyzed ¹³C-NC/aqueous cuprammonium solution. ¹³C NMR revealed that the labeling ratio of ¹³C to ¹²C in ¹³C-labeled cellulose was 48.3% (mol/mol). The degree of in vivo decomposition is defined as the ratio of ¹³C in ¹³CO₂ in the expired gas from mice to ¹³C in ¹³C-labeled cellulose fed to the mice. Although the decomposition rate for regenerated cellulose by enzymatic saccharification was markedly higher than that for natural cellulose under in vitro conditions, the decomposition of regenerated cellulose by mice was similar to that for natural cellulose under in vivo conditions, with decomposition rates of 18.1% (w/w) for regenerated cellulose and 15.4% (w/w) for natural cellulose. These results suggest the potential of regenerated cellulose as a low-calorie food material similar to natural cellulose.

中文翻译：

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小鼠体内13 C标记纤维素的体内分解

摘要

预期在市场上可以买到再生纤维素的食品，这是首次研究了小鼠消化器官中再生纤维素的体内分解。在过去的研究中，在标记的CO ₂环境中从种子中生长出植物，然后从这些植物中提取标记的纤维素。所获得的少量标记的纤维素使得难以在消化道中进行纤维素的体内分解研究。在本研究中，开发了一种新方法来克服这一问题。¹³ C标记的天然纤维素（¹³ C-NC）是由补充了¹³ C标记的葡萄糖的木糖葡糖杆菌培养物产生的。¹³然后由水解的¹³ C-NC /铜铵水溶液制备C标记的再生纤维素。¹³ C NMR显示在¹³ C标记的纤维素中¹³ C至¹² C的标记率为48.3％（mol / mol）。在体内分解的程度被定义为比率¹³下在¹³ CO ₂从小鼠的呼出气体中的¹³用C ¹³用C标记的纤维素喂养小鼠。尽管在体外条件下酶促糖化对再生纤维素的分解率明显高于天然纤维素，但小鼠在体内条件下对再生纤维素的分解与天然纤维素相似，分解率为18.1％（w / w）（对于再生纤维素）和15.4％（w / w）（对于天然纤维素）。这些结果表明，再生纤维素作为类似于天然纤维素的低热量食品原料的潜力。