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DNA nanosheet as an excellent fluorescence anisotropy amplification platform for accurate and sensitive biosensing.
Talanta ( IF 5.6 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.talanta.2020.120730 Yu Xin Liu 1 , Xue Xiao 2 , Chun Hong Li 3 , Chen Men 1 , Qi Chao Ye 1 , Wen Yi Lv 1 , Yuan Fang Li 1 , Cheng Zhi Huang 4 , Shu Jun Zhen 1
Talanta ( IF 5.6 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.talanta.2020.120730 Yu Xin Liu 1 , Xue Xiao 2 , Chun Hong Li 3 , Chen Men 1 , Qi Chao Ye 1 , Wen Yi Lv 1 , Yuan Fang Li 1 , Cheng Zhi Huang 4 , Shu Jun Zhen 1
Affiliation
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Recently, various inorganic nanomaterials have been used as fluorescence anisotropy (FA) enhancers for biosensing successfully. However, most of them are size-uncontrollable and possess an intensive fluorescence quenching ability, which will seriously reduce the accuracy and sensitivity of FA method. Herein, we report a two-dimensional DNA nanosheet (DNS) without fluorescence quenching effect as a novel FA amplification platform. In our strategy, fluorophore-labeled probe DNA (pDNA) is linked onto the DNS surface through the hybridization with the handle DNA (hDNA) that extended from the DNS, resulting in the significantly enhanced FA value. After the addition of target, the pDNA was released from the DNS surface due to the high affinity between the hDNA and target, and the FA was decreased. Thus, target could be detected by the significantly decreased FA value. The linear range was 10-50 nM and the limit of detection was 8 nM for the single-stranded DNA detection. This new method is general and has been also successfully applied for the detection of ATP and thrombin sensitively. Our method improved the accuracy of FA assay and has great potential to detect series of biological analytes in complex biosensing systems.
中文翻译：
DNA纳米片作为出色的荧光各向异性扩增平台，可进行准确而灵敏的生物传感。
最近，各种无机纳米材料已经成功地用作荧光各向异性（FA）增强剂，用于生物传感。然而，它们中的大多数是尺寸不可控的，并且具有强烈的荧光猝灭能力，这将严重降低FA方法的准确性和灵敏度。在本文中，我们报道了没有荧光猝灭作用的二维DNA纳米片（DNS）作为新型FA扩增平台。在我们的策略中，荧光团标记的探针DNA（pDNA）通过与从DNS延伸出来的手柄DNA（hDNA）杂交而连接到DNS表面，从而显着提高了FA值。加入靶标后，由于hDNA与靶标之间的高亲和力，pDNA从DNS表面释放出来，FA降低。从而，通过显着降低的FA值可以检测到目标。线性范围是10-50 nM，单链DNA检测的检测极限是8 nM。这种新方法是通用的，也已成功地应用于灵敏地检测ATP和凝血酶。我们的方法提高了FA测定的准确性，并且在复杂的生物传感系统中具有检测一系列生物分析物的巨大潜力。
更新日期：2020-01-09
中文翻译：
DNA纳米片作为出色的荧光各向异性扩增平台，可进行准确而灵敏的生物传感。
最近，各种无机纳米材料已经成功地用作荧光各向异性（FA）增强剂，用于生物传感。然而，它们中的大多数是尺寸不可控的，并且具有强烈的荧光猝灭能力，这将严重降低FA方法的准确性和灵敏度。在本文中，我们报道了没有荧光猝灭作用的二维DNA纳米片（DNS）作为新型FA扩增平台。在我们的策略中，荧光团标记的探针DNA（pDNA）通过与从DNS延伸出来的手柄DNA（hDNA）杂交而连接到DNS表面，从而显着提高了FA值。加入靶标后，由于hDNA与靶标之间的高亲和力，pDNA从DNS表面释放出来，FA降低。从而，通过显着降低的FA值可以检测到目标。线性范围是10-50 nM，单链DNA检测的检测极限是8 nM。这种新方法是通用的，也已成功地应用于灵敏地检测ATP和凝血酶。我们的方法提高了FA测定的准确性，并且在复杂的生物传感系统中具有检测一系列生物分析物的巨大潜力。
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