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A sperm-cell-based biosensor using a fluorescence probe for responsive signal readout toward bitter flavor detection.
Talanta ( IF 5.6 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.talanta.2020.120731
Yulan Tian 1 , Ping Zhu 1 , Yating Chen 1 , Wei Chen 1 , Liping Du 1 , Chunsheng Wu 1 , Ping Wang 2
Affiliation  

Bitter flavor detection has attracted extensive attention in industry and basic research due to pharmacological and food safety issues. Opportunities exist to extend the conventional methods of bitter flavor evaluation in performance and operation. This study proposes a novel sperm-cell-based biosensor (SCB) that utilizes living mouse spermatids as the primary sensing element, employs Fluo 4-AM as a transducer and works in conjunction with flow cytometry to realize the rapid quantitative detection of bitter compounds. The preparation conditions of the SCB were optimized with different quinine concentrations, and quinine and two other bitter compounds were employed to verify the sensing properties. Furthermore, the responses of the SCB to five basic flavor types were characterized to evaluate the sensor specificity. The SCB enabled preliminary classification of three bitter substances by using principal component analysis (PCA). The results revealed that the SCB is convenient, inexpensive and easy to use and can respond to bitter compounds in a dose-dependent manner with high sensitivity, high specificity and a low limit of detection, providing a novel and efficient approach for comprehensive evaluation of bitter substances in many fields, such as the pharmaceutical and food industries and in biosafety.

中文翻译:

一种基于精子细胞的生物传感器,使用荧光探针对苦味检测做出响应信号读出。

由于药理学和食品安全问题,苦味检测在工业和基础研究中已引起广泛关注。存在扩展性能和操作中苦味评估的常规方法的机会。这项研究提出了一种新型的基于精子细胞的生物传感器(SCB),该传感器利用活的小鼠精子作为主要传感元件,采用Fluo 4-AM作为换能器,并与流式细胞仪结合使用,以实现对苦味化合物的快速定量检测。用不同的奎宁浓度优化了SCB的制备条件,并采用奎宁和其他两种苦味化合物来验证其传感性能。此外,表征了SC​​B对五种基本风味类型的响应,以评估传感器的特异性。SCB通过使用主成分分析(PCA)可以对三种苦味物质进行初步分类。结果表明,SCB方便,廉价且易于使用,并且能够以剂量依赖的方式响应苦味化合物,具有高灵敏度,高特异性和低检测限,为综合评价苦味提供了新颖有效的方法许多领域的物质,例如制药和食品工业以及生物安全。
更新日期:2020-01-09
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