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Fluorescent nanosensor designing via hybrid of carbon dots and post-imprinted polymers for the detection of ovalbumin.
Talanta ( IF 5.6 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.talanta.2020.120727
Xiaoyan Wang 1 , Shuangmei Yu 2 , Jiangru Wang 3 , Jialuo Yu 3 , Maryam Arabi 3 , Longwen Fu 3 , Bowei Li 3 , Jinhua Li 3 , Lingxin Chen 4
Affiliation  

We reported a facile strategy to assemble a ratiometric nanosensor for the ovalbumin (OVA) fluorescence determination and meanwhile it can be utilized for selective visual identification by naked eyes with fluorescent test papers under 365 nm UV lamp. The nanosensor was prepared through simply mixing blue color carbon dots (CDs) and green color core-shell imprinted polymers. Blue CDs were used directly as the internal reference without participating in the imprinting process and modified molecularly imprinted polymers (MIPs) were synthesized by post-imprinting, using fluorescein isothiocyanate (FITC) as fluorescence enhanced signal. Upon the addition of different concentrations of OVA, the fluorescence intensity of FITC was enhanced, while the fluorescence intensity of CDs was almost unchanged, leading to a detection limit as low as 15.4 nM. Accordingly, the fluorescence color was gradually changed from blue to dark olive green to green with naked eyes observation. Moreover, the ratiometric nanosensor was successfully applied to detect OVA in the human urine samples with satisfactory recoveries attaining of 92.0-104.0% with relative standard deviation (RSD) of 3.3-3.9% and 93.3-101.0% with RSDs of 2.7-3.8% for the spiked chicken egg white samples. This strategy reported here opens a novel pathway for biomacromolecule detection in real applications and can realize the visual observation on fluorescent test papers.

中文翻译:

荧光纳米传感器通过碳点和后印迹聚合物的混合体设计,用于检测卵白蛋白。

我们报道了一种简便的策略,该方法可以组装用于卵白蛋白(OVA)荧光测定的比率式纳米传感器,同时可以将其用于在365 nm紫外线灯下用荧光试纸通过肉眼进行选择性视觉识别。纳米传感器是通过简单地混合蓝色碳点(CD)和绿色核壳印迹聚合物制备的。蓝色CD被直接用作内部参考,而没有参与印迹过程,并且使用异硫氰酸荧光素(FITC)作为荧光增强信号,通过后印迹合成了改性的分子印迹聚合物(MIP)。加入不同浓度的OVA后,FITC的荧光强度增强,而CD的荧光强度几乎不变,导致检测限低至15.4 nM。因此,通过肉眼观察,荧光颜色从蓝色逐渐变为深橄榄绿色至绿色。此外,该比例纳米传感器已成功应用于人类尿液样品中的OVA检测,其回收率分别为92.0-104.0%,相对标准偏差(RSD)为3.3-3.9%和93.3-101.0%,RSD为2.7-3.8%。加标的鸡蛋白样品。本文报道的该策略为实际应用中的生物大分子检测开辟了一条新途径,并可以在荧光试纸上实现视觉观察。对于加标的鸡蛋白样品,相对标准偏差(RSD)为3.3-3.9%的0%和93.3-101.0%,相对标准偏差为2.7-3.8%。本文报道的该策略为实际应用中的生物大分子检测开辟了一条新途径,并可以在荧光试纸上实现视觉观察。对于加标的鸡蛋白样品,相对标准偏差(RSD)为3.3-3.9%的0%和93.3-101.0%,相对标准偏差为2.7-3.8%。本文报道的该策略为实际应用中的生物大分子检测开辟了一条新途径,并可以在荧光试纸上实现视觉观察。
更新日期:2020-01-09
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