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Small size nanoparticles-Co3O4 based lateral flow immunoassay biosensor for highly sensitive and rapid detection of furazolidone.
Talanta ( IF 5.6 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.talanta.2020.120729
Lihong Su 1 , Lulu Wang 1 , Xiaolin Yao 1 , Xuechi Yin 1 , Han Zhang 1 , Man Zhao 1 , Sijie Liu 1 , Zonghan Wang 1 , Jianlong Wang 1 , Daohong Zhang 1
Affiliation  

Lateral flow immunoassay (LFIA) biosensor is a paper-based tool and widely utilized in various fields. Here, we developed a novel LFIA biosensor by introducing Co3O4 nanoparticles (NPs) as signal labels for highly sensitive detection of 3-amino-2-oxazolidinone (AOZ), a metabolite of furazolidone. The characteristic brown color of Co3O4 NPs enabled AOZ to be visually detected by the LFIA. Significantly, the size of Co3O4 NPs is relatively small compared with most of other signal labels, which could remarkably reduce steric hindrance, increase immunoreaction probability and shorten the analysis time. Under optimal conditions, the novel Co3O4 NPs-LFIA could possess high sensitivity for the detection of AOZ with a detection limit of 0.4 ng mL-1 by naked eyes, which was at least 3-fold improved than that of the conventional gold nanoparticles (GNPs) based LFIA. Moreover, the detection could be achieved within 6 min and without cross-reactions with other analogue small molecules. Taking merits of convenience, rapid and sensitivity, the proposed Co3O4 NPs-LFIA may be readily adapted for the detection of other small molecules.

中文翻译:

基于小颗粒纳米颗粒-Co3O4的侧向流免疫测定生物传感器,用于高灵敏度和快速检测呋喃唑酮。

横向流动免疫测定(LFIA)生物传感器是一种基于纸张的工具,已广泛应用于各个领域。在这里,我们通过引入Co3O4纳米颗粒(NPs)作为信号标记物以高灵敏度检测3-氨基-2-恶唑烷酮(AOZ)(一种呋喃唑酮的代谢物),开发了一种新型的LFIA生物传感器。Co3O4 NP的特征性棕色使LFIA可以直观地检测到AOZ。值得注意的是,与大多数其他信号标记相比,Co3O4 NP的尺寸相对较小,这可以显着降低空间位阻,增加免疫反应的可能性并缩短分析时间。在最佳条件下,新型Co3O4 NPs-LFIA可以高度灵敏地检测AOZ,肉眼检测极限为0.4 ng mL-1,相比基于LFIA的常规金纳米颗粒(GNP)至少提高了3倍。此外,检测可以在6分钟内完成,并且不会与其他类似的小分子发生交叉反应。利用方便,快速和灵敏的优点,建议的Co3O4 NPs-LFIA可以很容易地用于检测其他小分子。
更新日期:2020-01-09
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