A fast-responsive fluorescent probe PI-Py was designed and synthesized for sensitive and selective detecting hypochlorite (ClO⁻). The probe PI-Py was intrinsically non-fluorescent due to the PET effect of the phenothiazine moiety. After reacting with ClO⁻, the PET effect was blocked, and a strong green emission of the BODIPY unit was recovered. The probe PI-Py showed an excellent selectivity towards ClO⁻ in comparison of other ROS. The limit of detection for ClO⁻ was calculated to be 1.7 nM, proving the probe's high sensitivity. Moreover, the probe PI-Py has been proved to be effective in visualizing the variations of exogenous ClO⁻ in living HeLa cells. A co-localization study employing Mito Tracker red reveals that the PI-Py is specifically located in the mitochondria.

一个快速响应性荧光探针PI-PY的设计和用于灵敏的和选择性检测次氯酸盐（CLO合成^- ）。由于吩噻嗪部分的PET效应，探针PI-Py本质上不发荧光。与CLO反应后^-中，PET作用被阻断，并回收BODIPY单元的强绿色发光。探针PI-PY表明朝向CLO优异的选择性^-在其他ROS的比较。检测CLO的极限^-经计算为1.7纳米，证明探头的高灵敏度。此外，探针P1-PY已被证明是有效的可视外源CLO的变化^-在活的HeLa细胞中。一项使用Mito Tracker red的共同定位研究表明，PI-Py特别位于线粒体中。