Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats.,Food & Function

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Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats.
Food & Function ( IF 5.1 ) Pub Date : 2020-01-24 , DOI: 10.1039/c9fo02306b
Snjezana Romic ₁ , Ana Djordjevic , Snezana Tepavcevic , Tijana Culafic , Mojca Stojiljkovic , Biljana Bursac , Jelena Stanisic , Milan Kostic , Ljupka Gligorovska , Goran Koricanac

Affiliation

Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.

中文翻译：

富含果糖的饮食和慢性应激对雄性大鼠心脏中胰岛素信号传导和糖原合酶激酶-3β和钠钾泵的调节作用。

富含果糖的饮食和长期的压力暴露均会引起代谢和心血管疾病。这项研究的目的是分别检查和检查富含果糖的饮食和慢性应激对胰岛素信号传导和调节心脏糖原合成和离子转运的分子的影响，并揭示这些影响是否与饮食中的变化相吻合。糖皮质激素受体（GR）激活。将雄性Wistar大鼠的饮用水中的果糖含量提高10％，和/或使其长期承受无法预料的压力，持续9周。胰岛素受体（IR），蛋白酪氨酸磷酸酶1B，胰岛素受体底物1（IRS1），蛋白激酶B（Akt），细胞外信号调节激酶1/2（ERK1 / 2）的蛋白表达和/或磷酸化，用蛋白质印迹法分析心脏组织中的糖原合酶激酶3β（GSK-3β）和Na + / K + -ATPaseα亚基。还分析了细胞质和核级分之间的GR分布。富含果糖的饮食可独立于压力降低pERK1 / 2（Thr202 / Tyr204）和pGSK-3β（Ser9）的水平，而长期应激会增加IRS1的含量并阻止果糖饮食引起的pAkt（Ser473）水平的降低。。富含果糖的饮食加上慢性应激会降低心脏IR的蛋白质含量，并减弱IRS1的上调。单独处理增加了Na + / K + -ATPaseα1-和α2-亚基的蛋白质含量，而联合处理后，α2含量处于对照水平，而α1含量低于对照水平。GR核积累的增加可能介导联合治疗对心脏IR和α2-亚基表达的影响。我们的研究提供了关于慢性应激以及果糖饮食和慢性应激结合对心脏中所研究分子的影响的新见解。

更新日期：2020-02-26

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