In the current research previously developed composites composed from poly (l-lactide) (PLLA) and nano-hydroxyapatite (10 wt% nHAp/PLLA) were functionalized with different concentrations of europium (III) (Eu³⁺). The aim of this study was to determine whether Eu³⁺ ions doped within the 10 wt% nHAp/PLLA scaffolds will improve the bioactivity of composites. Therefore, first set of experiments was designed to evaluate the effect of Eu³⁺ ions on morphology, viability, proliferation and metabolism of progenitor cells isolated from adipose tissue (hASC). Three different concentration were tested i.e. 1 mol%, 3 mol% and 5%mol. We identified the 10 wt% nHAp/[email protected] mol% Eu³⁺ scaffolds as the most cytocompatible. Further, we investigated the influence of the composites doped with 3 mol% Eu³⁺ ions on differentiation of hASC toward bone and cartilage forming cells. Our results showed that 10 wt% nHAp/[email protected] mol% Eu³⁺ scaffolds promotes osteogenesis and chondrogenesis of hASCs what was associated with improved synthesis and secretion of extracellular matrix proteins specific for bone and articular cartilage tissue. We also proved that obtained biomaterials have bio-imaging function and their integration with bone can be monitored using micro computed tomography (μCT).

在当前的研究中，先前开发的由聚（l-丙交酯）（PLLA）和纳米羟基磷灰石（10 wt％nHAp / PLLA）组成的复合材料用不同浓度的（（III）（Eu ³⁺）功能化。这项研究的目的是确定掺入10 wt％nHAp / PLLA支架中的Eu ³⁺离子是否会改善复合材料的生物活性。因此，设计了第一组实验以评估Eu ³⁺离子对分离自脂肪组织（hASC）的祖细胞的形态，活力，增殖和代谢的影响。测试了三种不同的浓度，即1mol％，3mol％和5％mol。我们确定了10 wt％nHAp / [受电子邮件保护] mol％Eu ³⁺支架是最与细胞相容的。此外，我们研究了掺杂3 mol％Eu ³⁺离子的复合材料对hASC向骨和软骨形成细胞分化的影响。我们的结果表明，10 wt％nHAp / [受电子邮件保护的] mol％Eu ³⁺支架可促进hASC的成骨和软骨生成，这与骨和关节软骨组织特异的细胞外基质蛋白的合成和分泌改善有关。我们还证明了所获得的生物材料具有生物成像功能，并且可以使用微型计算机断层扫描（μCT）监控其与骨骼的整合。