The passive particle agglutination (PA) test, once widely used for Mycoplasma pneumoniae (M. pneumoniae) antibody detection, has gradually been replaced by quantitative enzyme-linked immunosorbent assays (ELISA). However, the lack of diagnostic criteria for quantitative ELISA M. pneumoniae-IgG (MP-IgG) and the low positive rates of ELISA M. pneumoniae-IgM (MP-IgM) limit the diagnostic value of ELISA for M. pneumoniae infection in adults. Here, the diagnostic value of quantitative ELISA MP-IgG was evaluated in adults with Mycoplasma pneumoniae pneumonia (MPP). The serum M. pneumoniae antibodies were detected in 162 patients with MPP, 228 patients with community-acquired pneumonia (CAP) with non-Mycoplasma pneumoniae (NMP), and 162 healthy controls by ELISA, using the PA results as the reference standards. For the MP-IgM-/IgG+ subgroup, a single serum MP-IgG level of ≥92.67 RU/mL can be used as a reference criterion for the diagnosis of acute M. pneumoniae infection. At admission, for patients with CAP, the sensitivity and specificity of ELISA MP-IgM positivity for MPP were 18.51% and 99.56%, respectively. MP-IgM positivity combined with MP-IgG ≥ 92.67 RU/mL increased the sensitivity to 40.12% and decreased the specificity to 94.29%. For paired serum samples obtained within seven days, an ELISA MP-IgG concentration change of ≥1.48-fold and MP-IgG ≥ 92.67 RU/mL on day 7 were used as the diagnostic criteria for M. pneumoniae infection. Accordingly, the combination of qualitative MP-IgM detection and quantitative MP-IgG detection by ELISA is valuable for acute MPP diagnosis in adults.

中文翻译：

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定量MP-IgG对成人肺炎支原体肺炎的诊断价值。

被动粒子凝集（PA）测试曾经广泛用于肺炎支原体（M. pneumoniae）抗体检测，现已逐渐被定量酶联免疫吸附测定（ELISA）取代。但是，缺乏定量ELISA肺炎支原体-IgG（MP-IgG）的诊断标准以及ELISA肺炎支原体-IgM（MP-IgM）的低阳性率限制了ELISA对成人肺炎支原体感染的诊断价值。在此，评估了定量ELISA MP-IgG对肺炎支原体肺炎（MPP）成人的诊断价值。使用PA结果作为参考标准，通过ELISA检测了162例MPP患者，228例社区获得性肺炎（CAP）和非支原体肺炎（NMP）患者以及162例健康对照的血清肺炎支原体抗体。对于MP-IgM- / IgG +子组，≥92.67 RU / mL的血清MP-IgG单一水平可作为诊断急性肺炎支原体感染的参考标准。入院时，CAP患者的ELISA MP-IgM阳性MPP敏感性和特异性分别为18.51％和99.56％。MP-IgM阳性与MP-IgG≥92.67 RU / mL结合可将敏感性提高到40.12％，将特异性降低到94.29％。对于在7天内获得的配对血清样品，将第7天的ELISA MP-IgG浓度变化≥1.48倍和MP-IgG≥92.67 RU / mL用作肺炎支原体感染的诊断标准。因此，通过ELISA进行定性MP-IgM检测和定量MP-IgG检测相结合，对于成人急性MPP诊断很有价值。肺炎感染。入院时，CAP患者的ELISA MP-IgM阳性MPP敏感性和特异性分别为18.51％和99.56％。MP-IgM阳性与MP-IgG≥92.67 RU / mL结合可将敏感性提高到40.12％，将特异性降低到94.29％。对于在7天内获得的配对血清样品，将第7天的ELISA MP-IgG浓度变化≥1.48倍和MP-IgG≥92.67 RU / mL用作肺炎支原体感染的诊断标准。因此，通过ELISA进行定性MP-IgM检测和定量MP-IgG检测相结合，对于成人急性MPP诊断很有价值。肺炎感染。入院时，CAP患者的ELISA MP-IgM阳性MPP敏感性和特异性分别为18.51％和99.56％。MP-IgM阳性与MP-IgG≥92.67 RU / mL结合可将敏感性提高到40.12％，将特异性降低到94.29％。对于在7天内获得的配对血清样品，将第7天的ELISA MP-IgG浓度变化≥1.48倍和MP-IgG≥92.67 RU / mL用作肺炎支原体感染的诊断标准。因此，通过ELISA进行定性MP-IgM检测和定量MP-IgG检测相结合，对于成人急性MPP诊断很有价值。67 RU / mL将敏感性提高到40.12％，而特异性降低到94.29％。对于在7天内获得的配对血清样品，将第7天的ELISA MP-IgG浓度变化≥1.48倍和MP-IgG≥92.67 RU / mL用作肺炎支原体感染的诊断标准。因此，通过ELISA进行定性MP-IgM检测和定量MP-IgG检测相结合，对于成人急性MPP诊断很有价值。67 RU / mL将敏感性提高到40.12％，而特异性降低到94.29％。对于在7天内获得的配对血清样品，将第7天的ELISA MP-IgG浓度变化≥1.48倍和MP-IgG≥92.67 RU / mL用作肺炎支原体感染的诊断标准。因此，通过ELISA进行定性MP-IgM检测和定量MP-IgG检测相结合，对于成人急性MPP诊断很有价值。