Organic solid waste is one of the most promising feedstocks for the implementation of the circular economy principles in waste management. Its anaerobic treatment can indeed promote organic matter conversion into a number of value-added products as well as energy carriers. However, the identification of sustainable strategies to handle organic solid waste in a biorefinery framework still poses technological as well as economic challenges. The aim of this study was in enhancing the potential of the organic fraction of municipal solid waste (OFMSW) to produce bioenergy and biochemicals by combining dark fermentation with a formic acid pretreatment. Hydrogen yields up to 31.6 ml/g_VS were obtained pre-treating the OFMSW with 5% formic acid, at 80 °C for 70 min. Concomitantly, a wide range of metabolites of market significance, including acetic acid, butyric acid and ethanol, accumulated. The concentration of these metabolites further enhanced after the dark fermentation of the pretreated substrates. Experimental tests highlighted the influence of the different pretreatment operating conditions on the relative production of hydrogen and main metabolites as well as the related pathways. It was found that the acid concentration plays a key role in promoting the biological conversion of OFMSW and that the adjustment of the operating temperature and treatment time can be targeted towards the production of either building blocks or energy carriers, so as to ensure the viability of the process for its scale up.

有机固体废物是在废物管理中实施循环经济原则的最有希望的原料之一。它的厌氧处理确实可以促进有机物转化为多种增值产品以及能源载体。然而，确定在生物精炼框架中处理有机固体废物的可持续策略仍然带来技术和经济挑战。这项研究的目的是通过将黑暗发酵与甲酸预处理相结合，提高城市固体废物（OFMSW）的有机部分产生生物能源和生化物质的潜力。氢气产量高达31.6 ml / g _VS在80°C下用5％甲酸对OFMSW进行预处理，得到的产物为70％。伴随地，积累了具有市场意义的多种代谢产物，包括乙酸，丁酸和乙醇。在预处理底物的黑暗发酵后，这些代谢物的浓度进一步提高。实验测试强调了不同预处理操作条件对氢和主要代谢物的相对产生以及相关途径的影响。已发现酸浓度在促进OFMSW的生物转化中起关键作用，并且可以将操作温度和处理时间的调整直接用于生产基石或能量载体，从而确保其生命力。扩大规模的过程。