The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty.,Cell Stem Cell

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The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty.
Cell Stem Cell ( IF 19.8 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.stem.2019.12.005
Jingtao Guo ₁ , Xichen Nie ₂ , Maria Giebler ₃ , Hana Mlcochova ₃ , Yueqi Wang ₄ , Edward J Grow ₂ , ₅ , Robin Kim ₆ , Melissa Tharmalingam ₇ , Gabriele Matilionyte ₇ , Cecilia Lindskog ₈ , Douglas T Carrell ₉ , Rod T Mitchell ₇ , Anne Goriely ₃ , James M Hotaling ₉ , Bradley R Cairns ₂

Affiliation

Howard Hughes Medical Institute, Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; The Andrology Laboratory, Department of Surgery (Andrology/Urology), Center for Reconstructive Urology and Men's Health, University of Utah Health Sciences Center, Salt Lake City, UT 84112, USA.
Howard Hughes Medical Institute, Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112, USA.
Radcliffe Department of Medicine, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX39DS, UK.
Department of Computer Science, Columbia University, New York, NY 10027, USA.
DonorConnect, Murray, UT 84107, USA.
Section of Transplantation, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
MRC Centre for Reproductive Health, The Queen's Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, UK; Royal Hospital for Children and Young People, Edinburgh EH91LF, UK.
Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala 751 85, Sweden.
The Andrology Laboratory, Department of Surgery (Andrology/Urology), Center for Reconstructive Urology and Men's Health, University of Utah Health Sciences Center, Salt Lake City, UT 84112, USA.

The human testis undergoes dramatic developmental and structural changes during puberty, including proliferation and maturation of somatic niche cells, and the onset of spermatogenesis. To characterize this understudied process, we profiled and analyzed single-cell transcriptomes of ∼10,000 testicular cells from four boys spanning puberty and compared them to those of infants and adults. During puberty, undifferentiated spermatogonia sequentially expand and differentiate prior to the initiation of gametogenesis. Notably, we identify a common pre-pubertal progenitor for Leydig and myoid cells and delineate candidate factors controlling pubertal differentiation. Furthermore, pre-pubertal Sertoli cells exhibit two distinct transcriptional states differing in metabolic profiles before converging to an alternative single mature population during puberty. Roles for testosterone in Sertoli cell maturation, antimicrobial peptide secretion, and spermatogonial differentiation are further highlighted through single-cell analysis of testosterone-suppressed transfemale testes. Taken together, our transcriptional atlas of the developing human testis provides multiple insights into developmental changes and key factors accompanying male puberty.

中文翻译：

人类青春期睾丸发育的动态转录细胞图谱。

人类睾丸在青春期经历巨大的发育和结构变化，包括体细胞生态位细胞的增殖和成熟，以及精子发生的开始。为了表征这个正在研究的过程，我们对四个青春期男孩的约 10,000 个睾丸细胞的单细胞转录组进行了分析和分析，并将它们与婴儿和成人的转录组进行了比较。在青春期，未分化的精原细胞在配子发生开始之前依次扩增和分化。值得注意的是，我们确定了间质细胞和肌样细胞的共同青春期前祖细胞，并确定了控制青春期分化的候选因子。此外，青春期前的支持细胞在青春期期间收敛到替代的单一成熟群体之前表现出两种不同的代谢谱不同的转录状态。通过对睾酮抑制的转女性睾丸的单细胞分析，进一步强调了睾酮在支持细胞成熟、抗菌肽分泌和精原细胞分化中的作用。总而言之，我们的人类睾丸发育转录图谱为男性青春期的发育变化和关键因素提供了多种见解。

更新日期：2020-01-09

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