Here, I recount some adventures that I and my colleagues have had over some 60 years since 1957 studying the effects of drugs and neurotransmitters on neuronal excitability and ion channel function, largely, but not exclusively, using sympathetic neurons as test objects. Studies include effects of centrally active drugs on sympathetic transmission; neuronal action and neuroglial uptake of GABA in the ganglia and brain; the action of muscarinic agonists on sympathetic neurons; the action of bradykinin on neuroblastoma-derived cells; and the identification of M-current as a target for muscarinic action, including experiments to determine its distribution, molecular composition, neurotransmitter sensitivity, and intracellular regulation by phospholipids and their hydrolysis products. Techniques used include electrophysiological recording (extracellular, intracellular microelectrode, whole-cell, and single-channel patch-clamp), autoradiography, messenger RNA and complementary DNA expression, antibody injection, antisense knockdown, and membrane-targeted lipidated peptides. I finish with some recollections about my scientific career, funding, and changes in laboratory life and pharmacology research over the past 60 years.

中文翻译：

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神经元，受体和通道。

在这里，我叙述了我和我的同事自1957年以来60多年来研究药物和神经递质对神经元兴奋性和离子通道功能的影响的大部分冒险经历，这些冒险主要但不限于使用交感神经元作为测试对象。研究包括中枢活性药物对交感神经传递的影响。神经节和脑中GABA的神经元作用和神经胶质摄取；毒蕈碱激动剂对交感神经元的作用；缓激肽对神经母细胞瘤衍生细胞的作用；以及鉴定M-current作为毒蕈碱作用的靶标，包括进行实验以确定其分布，分子组成，神经递质敏感性以及磷脂及其水解产物对细胞内的调节作用。使用的技术包括电生理记录（细胞外，细胞内微电极，全细胞和单通道膜片钳），放射自显影，信使RNA和互补DNA表达，抗体注射，反义敲除和膜靶向脂化肽。最后，我回顾了过去60年来我的科学职业，资金以及实验室生活和药理学研究的变化。