Impact of vaccination during pregnancy and staphylococci concentration on the presence of Bacillus cereus in raw human milk,Journal of Perinatology

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Impact of vaccination during pregnancy and staphylococci concentration on the presence of Bacillus cereus in raw human milk
Journal of Perinatology ( IF 2.4 ) Pub Date : 2020-01-09 , DOI: 10.1038/s41372-019-0586-4
Veronique Demers-Mathieu ₁ , Gabrielle Mathijssen ₁ , Shawn Fels ₁ , Donald H Chace ₁ , Elena Medo ₁

Affiliation

Objective

This study aimed to determine whether vaccination during pregnancy, prematurity, and staphylococci concentration influenced the presence of B. cereus or staphylococcal enterotoxins (SEs) in raw human milk from healthy mothers.

Study design

Human milk samples were collected from 152 healthy women. B. cereus, S. aureus and coagulase-negative staphylococci (CNS) were enumerated using selective agar culture media. The detection of B. cereus spores and SEs were determined using ELISA.

Results

CNS and B. cereus concentrations in milk from non-vaccinated mothers were higher than that from mothers vaccinated during pregnancy, but S. aureus did not differ. Prematurity did not affect B. cereus or staphylococci in human milk. S. aureus and CNS concentrations in human milk with the presence of B. cereus were higher than that with the absence of B. cereus. Viable B. cereus was present in 9.2% of raw human milk samples whereas SEs were not detected in any samples.

Conclusions

Vaccination during pregnancy and low concentration of staphylococci could reduce the risk of B. cereus in raw human milk. The screening of B. cereus in raw human milk must be performed before pasteurization to reduce the risk of B. cereus infection in preterm infants.

中文翻译：

怀孕期间接种疫苗和葡萄球菌浓度对生母乳中蜡样芽孢杆菌存在的影响

客观的

本研究旨在确定怀孕期间的疫苗接种、早产和葡萄球菌浓度是否会影响健康母亲生母乳中蜡状芽孢杆菌或葡萄球菌肠毒素 (SEs) 的存在。

学习规划

从 152 名健康女性中收集人乳样本。使用选择性琼脂培养基对蜡状芽孢杆菌、金黄色葡萄球菌和凝固酶阴性葡萄球菌 (CNS) 进行计数。使用 ELISA 测定蜡状芽孢杆菌孢子和 SE 的检测。

结果

来自未接种疫苗的母亲的乳汁中的CNS 和B. cereus浓度高于怀孕期间接种疫苗的母亲的浓度，但S. aureus没有差异。早产不影响人乳中的蜡状芽孢杆菌或葡萄球菌。存在蜡状芽孢杆菌的人乳中金黄色葡萄球菌和中枢神经系统的浓度高于不存在蜡状芽孢杆菌的人乳中的金黄色葡萄球菌和中枢神经系统浓度。9.2% 的生母乳样品中存在活的蜡状芽孢杆菌，而在任何样品中均未检测到SE 。