Abstract From simple 2-dimensional (2D) structures to highly complex 3-dimensional (3D) structures, DNA is widely used as a building block material. Despite this advancement, mass production of DNA nanostructures was a challenge due to the high cost that accompanies the preparation of diverse DNA strands. Rolling circle amplification (RCA) is one of the cost-effective methods for the mass production of repetitive DNA structures without using an expensive laboratory thermal cycler. Here, we report a DNA hydrogel containing various single-stranded DNA nanostructures (Y, X, Tetrahedron) in a one-pot system, prepared by RCA, and their selective separation with various restriction enzymes. Each DNA nanostructure in the DNA hydrogel contained distinctive restriction sites that were responsive to different kinds of restriction enzymes, achieving selective separation of Y-, X- and Tetrahedron-shaped DNA structures. This study has the potential to contribute towards DNA-based biomedical applications by employing mass production and precisely controlled release of DNA nanostructures.

中文翻译：

---

从 DNA 水凝胶中选择性释放 DNA 纳米结构

摘要 从简单的二维 (2D) 结构到高度复杂的 3 维 (3D) 结构，DNA 被广泛用作积木材料。尽管取得了这一进步，但由于制备不同 DNA 链的成本高昂，因此 DNA 纳米结构的大规模生产仍是一项挑战。滚环扩增 (RCA) 是一种无需使用昂贵的实验室热循环仪即可大规模生产重复 DNA 结构的经济高效的方法。在这里，我们报告了一种由 RCA 制备的单锅系统中包含各种单链 DNA 纳米结构（Y、X、四面体）的 DNA 水凝胶，以及它们与各种限制性内切酶的选择性分离。DNA 水凝胶中的每个 DNA 纳米结构都包含独特的限制性位点，这些位点对不同种类的限制性内切酶有反应，实现 Y 形、X 形和四面体形 DNA 结构的选择性分离。这项研究有可能通过大规模生产和精确控制 DNA 纳米结构的释放，为基于 DNA 的生物医学应用做出贡献。