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Conversion and Stability of New Metabolites of Paralytic Shellfish Toxins under Different Temperature and pH Conditions.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b07063 Yijia Che 1 , Ling Ding 1 , Jiangbing Qiu 1 , Ying Ji 1 , Aifeng Li 1, 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b07063 Yijia Che 1 , Ling Ding 1 , Jiangbing Qiu 1 , Ying Ji 1 , Aifeng Li 1, 2
Affiliation
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A number of new C-11 hydroxyl metabolites (so-called M-toxins) of paralytic shellfish toxins (PSTs) have been discovered in contaminated shellfish, and trace amounts have also been detected in some strains of PST-producing microalgae. To investigate the chemical conversion and stability of M-toxins, mussel extracts were purified with solid-phase extraction cartridges (Oasis HLB) and Biogel P-2 resin columns and four partially purified M-toxin fractions were stored at different temperatures (-20, 4, and 20 °C) and pH values (3, 4, and 5). The concentrations and profiles of M-toxins in these fractions were analyzed using liquid chromatography coupled with tandem mass spectrometry for 27 weeks. Results further confirmed the chemical conversion pathway M1 → M3 → M5 and determined for the first time two new transformation pathways: M2 → M4 → M6 and neosaxitoxin (NEO) → M10. The half-lives of M1, M2, M4, and M10 were calculated using a first-order degradation kinetics model, which indicated that the degradation of all M-toxins was dependent upon the temperature and pH, increasing with rising temperature and pH. In comparison to M4 and M10, M1 was more sensitive to the temperature, followed by M2. Results suggest that M-toxins should be maintained at a low temperature (-20 °C) and low pH (3) for their prolonged storage. M-toxins were less stable than all of the common analogues of PSTs, which may be beneficial for shellfish to achieve rapid detoxification through transformation of PSTs to M-toxins. These new findings are of significance because they enable further understanding of the metabolism of PSTs and their detoxification mechanisms in contaminated shellfish.
中文翻译：
不同温度和pH条件下麻痹性贝类毒素新代谢产物的转化和稳定性。
在受污染的贝类中发现了许多新的麻痹性贝类毒素（PST）的C-11羟基代谢产物（所谓的M毒素），并且在某些产PST的微藻菌株中还检测到痕量。为了研究M毒素的化学转化和稳定性，贻贝提取物用固相萃取柱（Oasis HLB）和Biogel P-2树脂柱进行了纯化，四个部分纯化的M毒素馏分在不同温度下保存（-20， 4和20°C）和pH值（3、4和5）。使用液相色谱-串联质谱分析了27周，分析了这些馏分中M毒素的浓度和分布。结果进一步证实了化学转化途径M1→M3→M5，并首次确定了两个新的转化途径：M2→M4→M6和新萨克毒素（NEO）→M10。使用一级降解动力学模型计算M1，M2，M4和M10的半衰期，这表明所有M毒素的降解均取决于温度和pH，并随温度和pH的升高而增加。与M4和M10相比，M1对温度更敏感，其次是M2。结果表明，M毒素应保持在低温（-20°C）和低pH（3）下，以便长时间储存。M毒素比所有PST的常见类似物都不稳定，这对于贝类通过将PST转化为M毒素实现快速排毒可能是有益的。这些新发现具有重要意义，因为它们使人们能够进一步了解被污染的贝类中PST的代谢及其解毒机制。
更新日期：2020-01-23
中文翻译：
不同温度和pH条件下麻痹性贝类毒素新代谢产物的转化和稳定性。
在受污染的贝类中发现了许多新的麻痹性贝类毒素（PST）的C-11羟基代谢产物（所谓的M毒素），并且在某些产PST的微藻菌株中还检测到痕量。为了研究M毒素的化学转化和稳定性，贻贝提取物用固相萃取柱（Oasis HLB）和Biogel P-2树脂柱进行了纯化，四个部分纯化的M毒素馏分在不同温度下保存（-20， 4和20°C）和pH值（3、4和5）。使用液相色谱-串联质谱分析了27周，分析了这些馏分中M毒素的浓度和分布。结果进一步证实了化学转化途径M1→M3→M5，并首次确定了两个新的转化途径：M2→M4→M6和新萨克毒素（NEO）→M10。使用一级降解动力学模型计算M1，M2，M4和M10的半衰期，这表明所有M毒素的降解均取决于温度和pH，并随温度和pH的升高而增加。与M4和M10相比，M1对温度更敏感，其次是M2。结果表明，M毒素应保持在低温（-20°C）和低pH（3）下，以便长时间储存。M毒素比所有PST的常见类似物都不稳定，这对于贝类通过将PST转化为M毒素实现快速排毒可能是有益的。这些新发现具有重要意义，因为它们使人们能够进一步了解被污染的贝类中PST的代谢及其解毒机制。
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