BACKGROUND Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogens like E.coli. However, the mechanism underlying how cortisol regulates E.coli-induced inflammatory response in bovine endometrial epithelial cells (BEEC) remains elusive. RESULTS Cortisol decreased the expressions of IL1β, IL6, TNF-α, IL8, and TLR4 mRNA in BEEC treated with LPS or heat-killed E.coli, but up-regulated these gene expressions in BEEC stimulated by live E.coli. CONCLUSION Cortisol exerted the anti-inflammatory action on LPS- or heat-killed E.coli-stimulated BEEC, but the pro-inflammatory action on live E.coli-induced BEEC.

中文翻译：

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皮质醇对LPS，热灭活的大肠杆菌或活的大肠杆菌刺激的牛子宫内膜上皮细胞中促炎基因表达的不同影响。

背景技术细菌感染在产后奶牛中很常见。已观察到围产期奶牛的皮质醇水平升高，并且与子宫内膜对诸如大肠杆菌的病原体的固有免疫力有关。但是，皮质醇如何调节牛子宫内膜上皮细胞（BEEC）中大肠杆菌诱导的炎症反应的机制尚不清楚。结果皮质醇降低了LPS或热灭性大肠杆菌处理的BEEC中IL1β，IL6，TNF-α，IL8和TLR4 mRNA的表达，但在活大肠杆菌刺激的BEEC中上调了这些基因的表达。结论皮质醇对LPS或热灭活的大肠杆菌刺激的BEEC具有抗炎作用，但对活的大肠杆菌诱导的BEEC具有促炎作用。