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Dynamic and regulated TAF gene expression during mouse embryonic germ cell development.
PLOS Genetics ( IF 4.0 ) Pub Date : 2020-01-08 , DOI: 10.1371/journal.pgen.1008515 Megan A Gura 1 , Maria M Mikedis 2 , Kimberly A Seymour 1 , Dirk G de Rooij 2 , David C Page 2, 3, 4 , Richard N Freiman 1
PLOS Genetics ( IF 4.0 ) Pub Date : 2020-01-08 , DOI: 10.1371/journal.pgen.1008515 Megan A Gura 1 , Maria M Mikedis 2 , Kimberly A Seymour 1 , Dirk G de Rooij 2 , David C Page 2, 3, 4 , Richard N Freiman 1
Affiliation
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Germ cells undergo many developmental transitions before ultimately becoming either eggs or sperm, and during embryonic development these transitions include epigenetic reprogramming, quiescence, and meiosis. To begin understanding the transcriptional regulation underlying these complex processes, we examined the spatial and temporal expression of TAF4b, a variant TFIID subunit required for fertility, during embryonic germ cell development. By analyzing published datasets and using our own experimental system to validate these expression studies, we determined that both Taf4b mRNA and protein are highly germ cell-enriched and that Taf4b mRNA levels dramatically increase from embryonic day 12.5-18.5. Surprisingly, additional mRNAs encoding other TFIID subunits are coordinately upregulated through this time course, including Taf7l and Taf9b. The expression of several of these germ cell-enriched TFIID genes is dependent upon Dazl and/or Stra8, known regulators of germ cell development and meiosis. Together, these data suggest that germ cells employ a highly specialized and dynamic form of TFIID to drive the transcriptional programs that underlie mammalian germ cell development.
中文翻译：
小鼠胚胎生殖细胞发育过程中的动态和受监管的TAF基因表达。
生殖细胞在最终成为卵子或精子之前经历了许多发育过渡，并且在胚胎发育过程中，这些过渡包括表观遗传重编程，静止和减数分裂。为了开始理解这些复杂过程的转录调控，我们研究了胚胎生殖细胞发育过程中TAF4b的时空表达，TAF4b是受精所需的变体TFIID亚基。通过分析已公开的数据集并使用我们自己的实验系统来验证这些表达研究，我们确定Taf4b mRNA和蛋白均高度富集生殖细胞，并且Taf4b mRNA水平从胚胎第12.5-18.5天起急剧增加。令人惊讶的是，编码其他TFIID亚基的其他mRNA在该时间过程中被协同上调，包括Taf71和Taf9b。这些富含生殖细胞的TFIID基因中的几种表达取决于Dazl和/或Stra8，这是生殖细胞发育和减数分裂的已知调节剂。总之，这些数据表明生殖细胞采用高度专业化和动态的TFIID形式来驱动构成哺乳动物生殖细胞发育基础的转录程序。
更新日期：2020-02-18
中文翻译：
小鼠胚胎生殖细胞发育过程中的动态和受监管的TAF基因表达。
生殖细胞在最终成为卵子或精子之前经历了许多发育过渡，并且在胚胎发育过程中，这些过渡包括表观遗传重编程，静止和减数分裂。为了开始理解这些复杂过程的转录调控，我们研究了胚胎生殖细胞发育过程中TAF4b的时空表达，TAF4b是受精所需的变体TFIID亚基。通过分析已公开的数据集并使用我们自己的实验系统来验证这些表达研究，我们确定Taf4b mRNA和蛋白均高度富集生殖细胞，并且Taf4b mRNA水平从胚胎第12.5-18.5天起急剧增加。令人惊讶的是，编码其他TFIID亚基的其他mRNA在该时间过程中被协同上调，包括Taf71和Taf9b。这些富含生殖细胞的TFIID基因中的几种表达取决于Dazl和/或Stra8，这是生殖细胞发育和减数分裂的已知调节剂。总之，这些数据表明生殖细胞采用高度专业化和动态的TFIID形式来驱动构成哺乳动物生殖细胞发育基础的转录程序。
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