Proteomic and Metabolomic Profilings Reveal Crucial Functions of γ-Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress.,Journal of Proteome Research

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Proteomic and Metabolomic Profilings Reveal Crucial Functions of γ-Aminobutyric Acid in Regulating Ionic, Water, and Metabolic Homeostasis in Creeping Bentgrass under Salt Stress.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-01-16 , DOI: 10.1021/acs.jproteome.9b00627
Zhou Li ₁ , Bizhen Cheng ₁ , Weihang Zeng ₁ , Xinquan Zhang ₁ , Yan Peng ₁

Affiliation

The global emergence of soil salinization poses a serious challenge to many countries and regions. γ-Aminobutyric acid (GABA) is involved in systemic regulation of plant adaptation to salt stress but the underlying molecular and metabolic mechanism still remains largely unknown. The elevated endogenous GABA level by the application of exogenous GABA improved salt tolerance associated with the enhancement of antioxidant capacity, photosynthetic characteristics, osmotic adjustment (OA), and water use efficiency in creeping bentgrass. GABA strongly upregulated transcript levels of AsPPa2, AsATPaB2, AsNHX2/4/6, and AsSOS1/20 in roots involved in enhanced capacity of Na+ compartmentalization and mitigation of Na+ toxicity in the cytosol. Significant downregulation of AsHKT1/4 expression could be induced by GABA in leaves in relation to maintenance of the significantly lower Na+ content and higher K+/Na+ ratio. GABA-depressed aquaporin expression and accumulation induced declines in stomatal conductance and transpiration, thereby reducing water loss in leaves during salt stress. For metabolic regulation, GABA primarily enhanced sugar and amino acid accumulation and metabolism, largely contributing to improved salt tolerance through maintaining OA and metabolic homeostasis. Other major pathways could be related to GABA-induced salt tolerance including increases in antioxidant defense, heat shock proteins, and myo-inositol accumulation in leaves. Integrative analyses of molecular, protein, metabolic, and physiological changes reveal systemic functions of GABA in regulating ionic, water, and metabolic homeostasis in nonhalophytic creeping bentgrass under salt stress.

中文翻译：

蛋白质组学和代谢组学分析揭示了γ-氨基丁酸在盐胁迫下调节ent草的离子，水和代谢稳态方面的重要作用。

全球土壤盐渍化的出现给许多国家和地区带来了严峻的挑战。γ-氨基丁酸（GABA）参与植物对盐胁迫的适应性系统调节，但其潜在的分子和代谢机制仍然很大程度上未知。通过施用外源GABA可以提高内源GABA的水平，从而提高盐分耐受性，同时提高抗氧化能力，光合特性，渗透调节（OA）和ing草的水分利用效率。GABA强烈上调了根中AsPPa2，AsATPaB2，AsNHX2 / 4/6和AsSOS1 / 20的转录水平，这与提高Na +分隔能力和缓解细胞质中Na +毒性有关。与维持明显较低的Na +含量和较高的K + / Na +比有关，GABA可以诱导叶片中AsHKT1 / 4表达的显着下调。GABA降低了水通道蛋白的表达和积累，导致气孔导度和蒸腾作用下降，从而减少了盐胁迫期间叶片的水分流失。对于代谢调节，GABA主要增强糖和氨基酸的积累与代谢，主要通过维持OA和代谢稳态来提高耐盐性。其他主要途径可能与GABA诱导的耐盐性有关，包括抗氧化防御能力的增强，热休克蛋白的增加以及叶片中肌醇的积累。分子，蛋白质，代谢和生理变化的综合分析揭示了GABA在调节离子，

更新日期：2020-01-17

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