Histopathological examination typically involves tissue resection or biopsy, fixation, sectioning, and staining protocols. A non-contact high-resolution photoacoustic remote sensing microscopy system is presented which is capable of depth-resolved imaging of cell nuclei in fixed and fresh tissues without the need for stains or labels. The reflection-mode system is based on a 0.5 numerical aperture reflective objective and enables fast optical scanning using a 600 kHz repetition rate fiber laser to produce histological-like images with 0.39 µm resolution and with close agreement to traditional H&E and fluorescence staining.

中文翻译：

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基于反射物镜的紫外光声遥感虚拟组织病理学

组织病理学检查通常涉及组织切除或活检，固定，切片和染色方案。提出了一种非接触式高分辨率光声遥感显微镜系统，该系统能够对固定和新鲜组织中的细胞核进行深度解析成像，而无需染色或标记。反射模式系统基于0.5数值孔径的反射物镜，并能够使用600 kHz重复频率光纤激光器进行快速光学扫描，以产生具有0.39 µm分辨率的组织学样图像，并且与传统的H＆E和荧光染色非常一致。