当前位置：
X-MOL 学术
›
Food Funct.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Analysis of the anti-fatigue activity of polysaccharides from Spirulina platensis: role of central 5-hydroxytryptamine mechanisms.
Food & Function ( IF 5.1 ) Pub Date : 2020-02-14 , DOI: 10.1039/c9fo02804h Meiju Zhu 1 , Hongzhu Zhu 1 , Xiaomin Ding 1 , Shaosheng Liu 1 , Yuanhua Zou 2
Food & Function ( IF 5.1 ) Pub Date : 2020-02-14 , DOI: 10.1039/c9fo02804h Meiju Zhu 1 , Hongzhu Zhu 1 , Xiaomin Ding 1 , Shaosheng Liu 1 , Yuanhua Zou 2
Affiliation
|
This study evaluated the effects of polysaccharides from Spirulina platensis (PSP) on endurance during treadmill exercise; levels of some biochemical indicators including hemoglobin (Hb), lactic acid (LA), creatine kinase (CK), and blood urea nitrogen (BUN); concentrations of 5-hydroxytryptamine (5-HT); and expressions of second isoforms of tryptophan hydroxylase (TPH2) and serotonergic type 1B inhibitory autoreceptor (5-HT1B) in the caudate putamen of exercising rats. Sixty Sprague-Dawley male rats were randomly divided into six groups: control group, exercise group, exercise and PSP (50, 100, or 200 mg kg-1)-treated groups, and exercise and caffeine (10 mg kg-1)-treated group (positive control). In the exercise groups, rats were put on a treadmill and forced to run for 30 min once a day for 6 consecutive days. On the 7th day of the experiment, time to exhaustion during the treadmill exercise was determined for the trained groups. Immediately after determination of the exhaustion time, all rats were sacrificed. Levels of Hb and LA were tested by the HiCN (Hemoglobin ferricyanide) colorimetry method and a colorimetric assay, respectively. Levels of CK and BUN were determined by automatic biochemical analyzer. 5-HT concentrations were detected by HPLC analysis. TPH2 and 5-HT1B expressions were measured by western blotting and real-time PCR. The results demonstrated that PSP prolonged the time to exhaustion during the treadmill exercise; increased Hb levels; decreased LA, BUN, and CK levels in the blood; suppressed the exercise-induced increase of 5-HT concentrations and TPH2 expression; and prevented the exercise-induced decrease of 5-HT1B expression in the caudate putamen. The most potent effects were observed at the PSP dose of 200 mg kg-1. These results suggest that the mechanism of promotion of physical performance by PSP might be related to increase in Hb levels; decrease in LA, BUN, and CK levels in the blood; inhibition of the exercise-induced 5-HT and TPH2 expressions; and the increase in the 5-HT1B expression in the caudate putamen of exercised rats.
中文翻译：
螺旋藻多糖的抗疲劳活性分析：中心5-羟色胺机制的作用。
这项研究评估了螺旋藻多糖（PSP）对跑步机运动耐力的影响。一些生化指标的水平，包括血红蛋白（Hb），乳酸（LA），肌酸激酶（CK）和血尿素氮（BUN）；5-羟色胺（5-HT）的浓度; 运动大鼠尾状壳中色氨酸羟化酶（TPH2）和血清素1B型抑制性自我受体（5-HT1B）的第二个同工型的表达 将60只Sprague-Dawley雄性大鼠随机分为六组：对照组，运动组，运动和PSP（50、100或200 mg kg-1）治疗组以及运动和咖啡因（10 mg kg-1）-治疗组（阳性对照）。在运动组中，将大鼠放在跑步机上，并每天连续60天每天跑步30分钟。在实验的第7天，确定训练有素的人群在跑步机锻炼过程中的疲劳时间。确定力竭时间后，立即处死所有大鼠。Hb和LA的水平分别通过HiCN（血红蛋白铁氰化物）比色法和比色测定法进行测试。CK和BUN的水平通过自动生化分析仪测定。通过HPLC分析检测5-HT浓度。通过Western印迹和实时荧光定量PCR检测TPH2和5-HT1B的表达。结果表明，PSP延长了跑步机锻炼中的疲劳时间。血红蛋白水平升高；降低血液中的LA，BUN和CK水平；抑制运动引起的5-HT浓度和TPH2表达增加；并阻止了运动引起的尾状壳中5-HT1B表达的降低。在200 mg kg-1的PSP剂量下观察到最有效的作用。这些结果表明，PSP促进身体机能的机制可能与血红蛋白水平的升高有关。血液中LA，BUN和CK含量降低；抑制运动诱导的5-HT和TPH2表达；和运动型大鼠尾状壳中5-HT1B表达的增加。
更新日期：2020-02-26
中文翻译：
螺旋藻多糖的抗疲劳活性分析：中心5-羟色胺机制的作用。
这项研究评估了螺旋藻多糖（PSP）对跑步机运动耐力的影响。一些生化指标的水平，包括血红蛋白（Hb），乳酸（LA），肌酸激酶（CK）和血尿素氮（BUN）；5-羟色胺（5-HT）的浓度; 运动大鼠尾状壳中色氨酸羟化酶（TPH2）和血清素1B型抑制性自我受体（5-HT1B）的第二个同工型的表达 将60只Sprague-Dawley雄性大鼠随机分为六组：对照组，运动组，运动和PSP（50、100或200 mg kg-1）治疗组以及运动和咖啡因（10 mg kg-1）-治疗组（阳性对照）。在运动组中，将大鼠放在跑步机上，并每天连续60天每天跑步30分钟。在实验的第7天，确定训练有素的人群在跑步机锻炼过程中的疲劳时间。确定力竭时间后，立即处死所有大鼠。Hb和LA的水平分别通过HiCN（血红蛋白铁氰化物）比色法和比色测定法进行测试。CK和BUN的水平通过自动生化分析仪测定。通过HPLC分析检测5-HT浓度。通过Western印迹和实时荧光定量PCR检测TPH2和5-HT1B的表达。结果表明，PSP延长了跑步机锻炼中的疲劳时间。血红蛋白水平升高；降低血液中的LA，BUN和CK水平；抑制运动引起的5-HT浓度和TPH2表达增加；并阻止了运动引起的尾状壳中5-HT1B表达的降低。在200 mg kg-1的PSP剂量下观察到最有效的作用。这些结果表明，PSP促进身体机能的机制可能与血红蛋白水平的升高有关。血液中LA，BUN和CK含量降低；抑制运动诱导的5-HT和TPH2表达；和运动型大鼠尾状壳中5-HT1B表达的增加。
京公网安备 11010802027423号