A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution.,Communications Biology

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A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution.
Communications Biology ( IF 5.2 ) Pub Date : 2020-01-08 , DOI: 10.1038/s42003-019-0742-6
Emma Gibbin ₁ , Guilhem Banc-Prandi _{2,

3} , Maoz Fine _{2,

3} , Arnaud Comment ₄ , Anders Meibom _{1,

5}

Affiliation

A wide range of organisms host photosynthesizing symbionts. In these animals the metabolic exchange between host and symbionts has prevented in situ host anabolic turnover to be studied without the confounding effect of translocated photosynthates. Using the symbiotic coral Stylophora pistillata as a model organism and [1-13C]-pyruvate and [2,3-13C]-pyruvate in different incubation conditions (light, light + DCMU, and darkness), we employed NanoSIMS isotopic imaging to quantify host anabolism, with and without translocated metabolites from their photosynthesizing dinoflagellate symbionts. Under our experimental conditions, host de novo lipid synthesis accounted for ~40% of the total holobiont lipid reserve, and dinoflagellate recycling of metabolic 13CO2 enhanced host tissue 13C-enrichment by 13-22% in the epidermis, 40-58% in the gastrodermis, and 135-169% in host lipid bodies. Furthermore, we show that host anabolic turnover in different tissue structures differs, in a manner consistent with the localisation, function and cellular composition of these structures.

中文翻译：

一种以亚细胞分辨率解开和定量光共生全生物中宿主同化代谢转换的方法。

各种各样的生物都可以进行光合作用。在这些动物中，宿主与共生体之间的代谢交换阻止了原位宿主合成代谢转换的研究，而没有易位的光合产物的混杂作用。使用共生珊瑚Stylophora pistillata作为模型生物，并在不同的孵化条件（光照，光照+ DCMU和黑暗）下使用[1-13C]-丙酮酸盐和[2,3-13C]-丙酮酸盐，我们使用NanoSIMS同位素成像进行定量宿主合成代谢，有或没有由其光合作用鞭毛状共生体产生的易位代谢物。在我们的实验条件下，新宿主的脂质合成约占总脂质总储备的40％，代谢鞭毛藻的13CO2的鞭毛藻再循环提高了表皮中13-22％的宿主组织13C富集，在胃中40-58％，和135-169％的宿主脂质体。此外，我们表明，在不同组织结构中的宿主合成代谢转换是不同的，其方式与这些结构的定位，功能和细胞组成一致。

更新日期：2020-01-08

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