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Employment of teicoplanin-coated magnetic particles for quantifying gram-positive bacteria via catalase-catalyzed hydrolysis reaction of H2O2.
Talanta ( IF 5.6 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.talanta.2020.120728 Yue Wu 1 , Shan Jiang 1 , Zhifeng Fu 1
Talanta ( IF 5.6 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.talanta.2020.120728 Yue Wu 1 , Shan Jiang 1 , Zhifeng Fu 1
Affiliation
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Based on the specific affinity behavior of teicoplanin towards Gram-positive (G+) bacteria, teicoplanin-coated magnetic particles were employed to separate G+ bacteria. Moreover, intracellular catalase released by catalase-positive bacteria led to catalyzed hydrolysis of H2O2 and inhibition of chemiluminescent signal of luminol-H2O2 system. By combining the separation capability of teicoplanin-coated magnetic particles and the inhibition of luminol chemiluminescence by catalase, a facile method was proposed for quantifying catalase-positive G+ bacteria. In this proof-of-principle work, Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus were adopted as model bacteria to evaluate its quantification performance. The three bacteria were quantified within the dynamic ranges of 5.0 × 102-5.0 × 107 CFU mL-1, 4.0 × 102-4.0 × 107 CFU mL-1 and 2.0 × 102-2.0 × 107 CFU mL-1, with detection limits of 156 CFU mL-1, 86 CFU mL-1 and 44 CFU mL-1, respectively. Gram-negative bacteria and catalase-negative G+ bacteria both displayed minor interference. The results for quantifying bacteria in milk, human urine and physiological saline samples demonstrated its reliability for real application. It provided a promising technique tool for food safety, medical diagnosis as well as drug quality control.
中文翻译：
使用过替考拉宁涂层的磁性颗粒通过过氧化氢酶催化的H2O2水解反应定量革兰氏阳性细菌。
基于替考拉宁对革兰氏阳性（G +）细菌的特异性亲和力行为，采用替考拉宁包覆的磁性颗粒分离G +细菌。此外，过氧化氢酶阳性细菌释放的细胞内过氧化氢酶导致H2O2的催化水解和Luminol-H2O2系统化学发光信号的抑制。通过结合替考拉宁包被的磁性颗粒的分离能力和过氧化氢酶对鲁米诺化学发光的抑制，提出了一种简便的定量过氧化氢酶阳性G +细菌的方法。在这项原理验证工作中，采用金黄色葡萄球菌，枯草芽孢杆菌和黄褐微球菌作为模型细菌，以评估其定量性能。在5.0×102-5.0×107 CFU mL-1、4.0×102-4的动态范围内对这三种细菌进行了定量。0×107 CFU mL-1和2.0×102-2.0×107 CFU mL-1，检测限分别为156 CFU mL-1、86 CFU mL-1和44 CFU mL-1。革兰氏阴性细菌和过氧化氢酶阴性G +细菌均显示出较小的干扰。牛奶，人尿和生理盐水样品中细菌定量的结果证明了其在实际应用中的可靠性。它为食品安全，医疗诊断以及药物质量控制提供了有前途的技术工具。
更新日期：2020-01-08
中文翻译：
使用过替考拉宁涂层的磁性颗粒通过过氧化氢酶催化的H2O2水解反应定量革兰氏阳性细菌。
基于替考拉宁对革兰氏阳性（G +）细菌的特异性亲和力行为，采用替考拉宁包覆的磁性颗粒分离G +细菌。此外，过氧化氢酶阳性细菌释放的细胞内过氧化氢酶导致H2O2的催化水解和Luminol-H2O2系统化学发光信号的抑制。通过结合替考拉宁包被的磁性颗粒的分离能力和过氧化氢酶对鲁米诺化学发光的抑制，提出了一种简便的定量过氧化氢酶阳性G +细菌的方法。在这项原理验证工作中，采用金黄色葡萄球菌，枯草芽孢杆菌和黄褐微球菌作为模型细菌，以评估其定量性能。在5.0×102-5.0×107 CFU mL-1、4.0×102-4的动态范围内对这三种细菌进行了定量。0×107 CFU mL-1和2.0×102-2.0×107 CFU mL-1，检测限分别为156 CFU mL-1、86 CFU mL-1和44 CFU mL-1。革兰氏阴性细菌和过氧化氢酶阴性G +细菌均显示出较小的干扰。牛奶，人尿和生理盐水样品中细菌定量的结果证明了其在实际应用中的可靠性。它为食品安全，医疗诊断以及药物质量控制提供了有前途的技术工具。
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