Construction of efficient “on-off-on” fluorescence aptasensor for ultrasensitive detection of prostate specific antigen via covalent energy transfer between g-C3N4 quantum dots and palladium triangular plates,Analytica Chimica Acta

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Construction of efficient “on-off-on” fluorescence aptasensor for ultrasensitive detection of prostate specific antigen via covalent energy transfer between g-C3N4 quantum dots and palladium triangular plates
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.aca.2020.01.009
Hui-Min Wang ₁ , Xiao-Qin Huang ₁ , Ai-Jun Wang ₁ , Xiliang Luo ₂ , Wei-Dong Liu ₁ , Pei-Xin Yuan ₁ , Jiu-Ju Feng ₁

Affiliation

Development of ultra-sensitive and high specific aptasensors is important for early diagnosis of prostate cancer. Herein, ultrasensitive detection of prostate specific antigen (PSA) aptasensor was realized based on the "on-off-on" model via fluorescence (FL) covalent energy transfer between g-C3N4 quantum dot (g-CNQDs) and palladium triangular plates (Pd TPs). Specifically, the Pd TPs were primarily linked with PSA aptamer (PA) as the reporter probe, followed by attaching them onto the g-CNQDs surfaces, causing the highly enlarged FL quenching rate (ca. 75%). After the introduction of PSA, the FL intensities recovered again because of the distinctively stronger affinity of PA to PSA than that of g-CNQDs. The bond of pyridine N with Pd was identified as efficient energy transfer pathway through the X-ray photoelectron spectroscopy (XPS) and FL measurements. The surface plasmon resonance (SPR) experiments certified the remarkably different affinity of PA towards g-CNQDs and PSA. The as-constructed FL aptasensor can accurately quantify PSA with wide linear range of 10 pg mL-1-50 ng mL-1 and ultra-low limit of detection (LOD, 4.2 pg mL-1), indicating the promising applications in clinical assay and biological detection.

中文翻译：

通过g-C3N4量子点和钯三角板之间的共价能量转移构建高效的“on-off-on”荧光适体传感器用于前列腺特异性抗原的超灵敏检测

超灵敏和高特异性适体传感器的开发对于前列腺癌的早期诊断具有重要意义。在此，基于“on-off-on”模型，通过g-C3N4量子点（g-CNQDs）和钯三角板（Pd）之间的荧光（FL）共价能量转移实现了前列腺特异性抗原（PSA）适体传感器的超灵敏检测。 TP）。具体而言，Pd TPs 主要与作为报告探针的 PSA 适体 (PA) 连接，然后将它们连接到 g-CNQDs 表面，导致高度增大的 FL 淬灭率（约 75%）。引入 PSA 后，由于 PA 对 PSA 的亲和力明显强于 g-CNQD，因此 FL 强度再次恢复。通过 X 射线光电子能谱 (XPS) 和 FL 测量，吡啶 N 与 Pd 的键被确定为有效的能量转移途径。表面等离子体共振 (SPR) 实验证明 PA 对 g-CNQD 和 PSA 的亲和力显着不同。所构建的 FL 适体传感器可以准确量化 PSA，线性范围为 10 pg mL-1-50 ng mL-1 和超低检测限（LOD，4.2 pg mL-1），表明在临床检测中的应用前景广阔和生物检测。

更新日期：2020-04-01

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