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Self-Validated Homogeneous Immunoassay by Single Nanoparticle in-Depth Scrutinization.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.analchem.9b05596 Zili Huang 1 , Chaoqun Wang 1 , Rui Liu 1 , Yingying Su 2 , Yi Lv 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.analchem.9b05596 Zili Huang 1 , Chaoqun Wang 1 , Rui Liu 1 , Yingying Su 2 , Yi Lv 2
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The most convenient method for the clinical routine analysis of disease biomarkers is homogeneous immunoassay, which minimizes the requirements for automation and time-/lab-consumption. Despite great success, because sample constituents are not removed by a separation or washing step, a major challenge in conducting homogeneous immunoassays for the practical application is the matrix effect-related inaccuracy. Herein, to guarantee an accurate quantification, a self-validated homogeneous immunoassay was proposed, by simultaneously scrutinizing both frequency and intensity of single gold nanoparticles. The two analytical modes of single particle inductively coupled plasma mass spectrometry (ICPMS) correlated well with each other, resulting in a self-validation mechanism for the accurate immunoassay. Both two modes of the proposed method provided linear ranges of 2 orders of magnitude and LODs of pM level. Thanks to the self-validated strategy and the high tolerance of the matrix effect of ICPMS, the proposed homogeneous immunoassay was successfully demonstrated in a series of human serum samples, with results in good accordance with clinical routine methods.
中文翻译：
通过单个纳米颗粒深入检查进行自我验证的均质免疫测定。
疾病生物标记物临床常规分析的最便捷方法是均相免疫测定，可将自动化和时间/实验室消耗量的要求降至最低。尽管取得了巨大的成功，但由于没有通过分离或洗涤步骤去除样品成分，因此在实际应用中进行均相免疫测定的主要挑战是与基质效应有关的不准确性。在此，为了保证准确的定量，提出了一种通过同时检查单个金纳米颗粒的频率和强度来进行自我验证的均质免疫测定方法。单粒子电感耦合等离子体质谱法（ICPMS）的两种分析模式相互之间具有很好的相关性，从而形成了一种用于精确免疫测定的自验证机制。所提出方法的两种模式均提供2个数量级的线性范围和pM级的LOD。归功于自我验证的策略和ICPMS基质效应的高度耐受性，该提议的均相免疫测定已成功在一系列人血清样品中得到证实，其结果与临床常规方法非常吻合。
更新日期：2020-01-17
中文翻译：
通过单个纳米颗粒深入检查进行自我验证的均质免疫测定。
疾病生物标记物临床常规分析的最便捷方法是均相免疫测定，可将自动化和时间/实验室消耗量的要求降至最低。尽管取得了巨大的成功，但由于没有通过分离或洗涤步骤去除样品成分，因此在实际应用中进行均相免疫测定的主要挑战是与基质效应有关的不准确性。在此，为了保证准确的定量，提出了一种通过同时检查单个金纳米颗粒的频率和强度来进行自我验证的均质免疫测定方法。单粒子电感耦合等离子体质谱法（ICPMS）的两种分析模式相互之间具有很好的相关性，从而形成了一种用于精确免疫测定的自验证机制。所提出方法的两种模式均提供2个数量级的线性范围和pM级的LOD。归功于自我验证的策略和ICPMS基质效应的高度耐受性，该提议的均相免疫测定已成功在一系列人血清样品中得到证实，其结果与临床常规方法非常吻合。
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