BACKGROUND Colistin resistance is considered a serious problem due to a lack of alternative antibiotics. The Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test is a resazurin reduction-based technique that relies on the visual detection of bacterial growth in the presence of a defined concentration of colistin. The aim of this study was to evaluate the performance of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test in the detection of colistin susceptibility in common clinical Gram-negative bacteria. RESULTS A total of 253 clinical isolates from a teaching hospital, including Acinetobacter baumanii (n = 58, 8 colistin-resistant), Pseudomonas aeruginosa (n = 61, 11 colistin-resistant), Klebsiella pneumoniae (n = 70, 20 colistin-resistant) and Escherichia coli (n = 64, 14 colistin-resistant) were tested in this study. The sensitivity and specificity of the Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test compared to Broth microdilution method was 100 and 99%, respectively. CONCLUSIONS Our results suggest that Rapid ResaPolymyxin Acinetobacter/Pseudomonas NP test could be used as an accurate detection method for colistin resistance.

中文翻译：

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评估基于刃天青的快速检测多粘菌抗性革兰氏阴性菌的方法。

背景技术由于缺乏替代抗生素，因此对科氏素的抗性被认为是一个严重的问题。快速ResaPolymyxin不动杆菌/假单胞菌NP测试是一种基于刃天青还原的技术，该技术依赖于在确定浓度的大肠菌素存在下视觉检测细菌的生长。这项研究的目的是评估快速ResaPolymyxin不动杆菌/假单胞菌NP测试在检测常见临床革兰氏阴性细菌中粘菌素敏感性方面的性能。结果来自教学医院的总共253株临床分离株，包括鲍曼不动杆菌（n = 58，对大肠菌素8耐药），铜绿假单胞菌（n = 61，对大肠菌素11耐药），肺炎克雷伯菌（n = 70，对大肠菌素20耐药） ）和大肠杆菌（n = 64，对大肠菌素14的抵抗力）进行了这项研究。与肉汤微量稀释法相比，快速ResaPolymyxin不动杆菌/假单胞菌NP检测的敏感性和特异性分别为100％和99％。结论我们的结果表明快速的ResaPolymyxin不动杆菌/假单胞菌NP检测可作为一种准确的检测大肠菌素耐药性的方法。