Both chitosan (CS) and poly(3-hydroxybutyrate) (P(3HB)) are two good options for use in tissue engineering. Some of the advantages of these polymers are biodegradability and non-toxicity. However, the inadequate mechanical properties and hydrophobicity (P3HB) and the low porosity (CS) are examples of shortcomings that restrict its applications. The polysaccharide was grafted onto P(3HB) in a previous work for removing these disadvantages. As of yet, it is still unknown whether our P(3HB)-g-CS has potential as an interface with biological systems. This work aimed to use the gamma radiation-induced grafted CS to prepare a polyurethane composite scaffold with silica nanoparticles obtained from vermicomposting and porosity induced by salt leaching. The obtained structural and morphological characterization, interconnectivity, degree of porosity and swelling, mechanical and morphometrical properties (μ-CT) showed that the new poly(saccharide-ester-urethane) composite properties are suitable for cell culture. The poly(urethane-urea) composites did not induce cytotoxic effects. Viability assays proved that it efficiently allows the three-dimensional culturing of pancreatic islet cells.

壳聚糖（CS）和聚（3-羟基丁酸酯）（P（3HB））都是用于组织工程的两个很好的选择。这些聚合物的一些优点是生物降解性和无毒性。但是，机械性能和疏水性（P3HB）不足以及低孔隙率（CS）不足是限制其应用的不足。在消除这些缺点的先前工作中，将多糖接枝到了P（3HB）上。到目前为止，我们的P（3HB）-g-CS是否具有作为与生物系统相互作用的接口的潜力仍然未知。这项工作旨在使用伽玛射线诱导的接枝CS制备具有二氧化硅纳米粒子的聚氨酯复合支架，该纳米粒子是通过ver积和盐浸诱导的孔隙度获得的。获得的结构和形态特征，互连性，孔隙度和溶胀度，力学性能和形态学性能（μ-CT）表明，新型的（糖-酯-氨基甲酸酯）复合材料性能适用于细胞培养。聚（氨基甲酸酯-脲）复合材料没有诱导细胞毒性作用。活力测定证明，它可以有效地进行胰岛细胞的三维培养。