Abstract The effects of gamma radiation were observed on the metamorphosis and reproduction as key target features to be intercepted for disinfestation of the three major pest bruchid species, Callosobruchus maculatus (Fabricius), C. analis (Fabricius) and C. chinensis (Linnaeus) irradiated in the various ontogenic stages. The present study was aimed to determine an efficacious generic dose of phytosanitary irradiation (PI) for bruchid disinfestation in pulses. Callosobruchus chinensis was the most radio-resistant species among the present tested species, and male bruchids were more radio-resistant than the females in this species complex. In this complex of tested bruchid species, the gamma dose required to cause 100% metamorphic disruption in the most radio-resistant pre-imaginal stage (pharate adult) was 20–46% higher than the dose required to induce complete sterility in the most radio-resistant imaginal stage (4–5 day old). The effective dose inducing 99.9% impact (ED99.9) was 105 Gy for sterility induction but ~181 Gy to induce metamorphic disruption in the most radio-resistant pre-imaginal pharate adult in C. chinensis, whereas the most radio-resistant imaginal stage (4–5 day old adult) of this species required 124 Gy for complete sterilization. Based on these results, 160–200 Gy as phytosanitary irradiation dose range was evaluated on a semi-large scale testing on the pigeonpea pulse seeds having mixed infestation of Callosobruchus species complex, which indicated that 200 Gy might be considered as a feasible generic dose of phytosanitary irradiation for bruchid disinfestation in pulses.

中文翻译：

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研究 Callosobruchus 物种复合体的个体放射敏感性，以建立植物检疫辐照的通用剂量作为豆类灭虫的收获后检疫处理

摘要 观察了伽马辐射对变态和繁殖的影响，作为对三种主要害虫 bruchid 物种，Callosobruchus maculatus (Fabricius)、C. analis (Fabricius) 和 C. chinensis (Linnaeus) 进行灭虫的关键目标特征。在各个个体发育阶段。本研究旨在确定一种有效的植物检疫辐照 (PI) 通用剂量，用于脉冲中的 bruchid 灭虫。Callosobruchus chinensis 是目前测试物种中抗辐射能力最强的物种，在该物种群中，雄性比雌性更抗辐射。在这个经过测试的 bruchid 物种的综合体中，在最抗辐射的成虫期（成虫成虫）中引起 100% 变质破坏所需的伽马剂量比在最抗辐射的成虫期（4–5）中诱导完全不育所需的剂量高 20–46%老天）。诱导 99.9% 影响 (ED99.9) 的有效剂量为 105 Gy 用于不育诱导，但 ~181 Gy 可诱导 C. chinensis 中最具抗辐射性的成虫前成虫的变质破坏，而最具抗辐射性的成虫阶段该物种（4-5 天大的成虫）需要 124 Gy 才能完全绝育。基于这些结果，对具有 Callosobruchus 物种复合体混合侵染的木豆豆类种子进行了半大规模试验，评估了 160-200 Gy 作为植物检疫辐照剂量范围，