Abstract Metarhizium genus is an important biological control agent. Most industrial conidia production processes are based on the use of plastic bags or containers containing ∼1 kg of substrate. Static bioreactors, such as packed columns, trays, and plastic bags, are used for different scale conidia production. However, the effect of aeration mechanism on conidia production and quality has not been evaluated. Therefore, the productivity and quality of conidia produced in three types of bioreactors were evaluated. M. robertsii strain Xoch 8.1 was used to determine the effect of temperature and airflow rate on conidia production and quality. The productivity of viable conidia in the packed column bioreactor was up to 1.8 and 1.5 times higher than that obtained with tray and bag bioreactors, respectively. Conidial infectivity was similar for the three types of bioreactors. Forced convection aeration in the packed columns resulted in higher levels of conidia production and productivity without affecting their quality. This study shows that forced aeration in tubular bioreactor allows the highest conidia production and productivity; additionally, the forced aeration allows the on-line monitoring of the production of CO2 during the fermentation process, as a tool to associate this measurement to conidiation patterns.

中文翻译：

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强制曝气促进固态发酵中罗伯氏绿僵菌的感染性分生孢子的高产量和生产力

摘要 绿僵菌属是重要的生物防治剂。大多数工业分生孢子生产过程是基于使用塑料袋或含有约 1 kg 基质的容器。静态生物反应器，如填充柱、托盘和塑料袋，用于不同规模的分生孢子生产。然而，尚未评估曝气机制对分生孢子生产和质量的影响。因此，对三种类型的生物反应器中产生的分生孢子的生产力和质量进行了评估。M. robertsii 菌株 Xoch 8.1 用于确定温度和气流速率对分生孢子生产和质量的影响。填充柱式生物反应器中活分生孢子的生产率分别比托盘和袋式生物反应器高出 1.8 倍和 1.5 倍。三种生物反应器的分生孢子传染性相似。填料塔中的强制对流曝气可在不影响质量的情况下提高分生孢子产量和生产力。该研究表明，管式生物反应器中的强制曝气可实现最高的分生孢子产量和生产力；此外，强制曝气允许在发酵过程中在线监测 CO2 的产生，作为将这种测量与分生孢子模式相关联的工具。