New strategies are being proposed in marine aquaculture to use marine bacteria as alternative to antibiotics, as nutritional additive or as immune-stimulant. These approaches are particularly promising for larval and juvenile cultures. In many cases, the bacteria are released in the seawater, where they have to be at appropriate concentrations. In addition, only low-cost technologies are sustainable for this industry, without any complex requirements for use or storage. In this work, we explore the possibilities of preservation of a potential marine probiotic bacterium (Phaeobacter PP-154) as a product suitable for use in marine aquaculture by addition to the seawater. A method which guaranteed the preservation of the viable marine bacteria in a saline medium and their rapid release in the seawater was searched for. In a previous step, classical procedures (freeze-drying and freezing) had been explored, but undesirable results of the interaction of the products obtained with natural seawater led to investigate alternatives. We report the results of the immobilization of the marine bacteria in calcium alginate beads. The final product complies the salinity which allows the requirements of the bacteria without interference with alginate in the formation of beads, and a balanced hardness to retain the bacteria and to be easily released in the marine aquaculture environment. The process was evaluated using the central composite rotatable design (CCRD), a standard response surface methodology (RSM).

中文翻译：

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封装用于水产养殖设施的海洋活细菌，并使用响应面方法进行过程评估。

在海水养殖中提出了新的策略，以使用海洋细菌替代抗生素，作为营养添加剂或作为免疫刺激剂。这些方法对于幼体和幼体培养特别有前途。在许多情况下，细菌会在海水中释放，必须在适当浓度下释放。另外，对于该行业而言，只有低成本技术才是可持续的，而对使用或存储没有任何复杂的要求。在这项工作中，我们探索了将潜在的海洋益生菌细菌（Phaeobacter PP-154）作为除海水外适用于海水养殖的产品的可能性。寻找一种方法，以确保能将活的海洋细菌保存在盐介质中并在海水中快速释放。在上一步中，已经探索了经典的程序（冷冻干燥和冷冻），但是获得的产品与天然海水相互作用的不良结果导致人们研究了替代方法。我们报告了海藻酸钙珠中海洋细菌固定化的结果。最终产品符合盐度要求，可满足细菌的要求，而不会干扰藻酸盐在珠粒形成中的作用，并具有平衡的硬度以保留细菌并易于在海水养殖环境中释放。使用中央复合材料可旋转设计（CCRD），标准响应面方法（RSM）对过程进行了评估。我们报告了海藻酸钙珠中海洋细菌固定化的结果。最终产品符合盐度要求，可满足细菌的要求，而不会干扰藻酸盐在珠粒形成中的作用，并具有平衡的硬度以保留细菌并易于在海水养殖环境中释放。使用中央复合材料可旋转设计（CCRD），标准响应面方法（RSM）对过程进行了评估。我们报告了海藻酸钙珠中海洋细菌固定化的结果。最终产品符合盐度要求，可满足细菌的要求，而不会干扰藻酸盐在珠粒形成中的作用，并具有平衡的硬度，可保留细菌并易于在海水养殖环境中释放。使用中央复合可旋转设计（CCRD），标准响应面方法（RSM）对过程进行了评估。