Abstract

The mounting threat of antibiotic-resistant bacterial infections has made it imperative to develop innovative antibacterial strategies. Here we propose a novel antibacterial nanoplatform of silver nanoparticles-decorated and mesoporous silica coated single-walled carbon nanotubes constructed via a N-[3-(trimethoxysilyl)propyl]ethylene diamine (TSD)-mediated method (SWCNTs@mSiO₂-TSD@Ag). In this system, the outer mesoporous silica shells are able to improve the dispersibility of SWCNTs, which will increase their contact area with bacteria cell walls. Meanwhile, the large number of mesopores in silica layers act as microreactors for in situ synthesis of Ag NPs with controlled small size and uniform distribution, which induces an enhanced antibacterial activity. Compared with TSD modified mesoporous silica coated single-walled carbon nanotubes (SWCNTs@mSiO₂-TSD) and commercial Ag NPs, this combination nanosystem of SWCNTs@mSiO₂-TSD@Ag exhibits much stronger antibacterial performance against multi-drug-resistant bacteria Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in vitro through damaging the bacterial cell membranes and a fast release of silver ions. Furthermore, the in vivo rat skin infection model verifies that SWCNTs@mSiO₂-TSD@Ag have remarkably improved abilities of bacterial clearance, wound healing promoting as well as outstanding biocompatibility. Therefore, this novel nanoplatform indicates promising potentials as a safe and powerful tool for the treatment of clinical drug-resistant infections.

中文翻译：

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装饰有银纳米颗粒且介孔二氧化硅涂层的单壁碳纳米管具有增强的抗菌活性，可杀死耐药细菌

摘要

抗生素耐药性细菌感染的威胁越来越大，因此必须开发创新的抗菌策略。在这里，我们提出了一种新型的抗菌纳米平台，该纳米平台是通过N- [3-（三甲氧基甲硅烷基）丙基]乙二胺（TSD）介导的方法（SWCNTs @ mSiO ₂ -TSD @ ）构建的纳米银修饰的介孔二氧化硅涂覆的单壁碳纳米管的抗菌纳米平台。Ag）。在该系统中，中孔二氧化硅壳能够改善SWCNT的分散性，这将增加它们与细菌细胞壁的接触面积。同时，二氧化硅层中大量的中孔充当了原位微反应器纳米银的合成具有受控的小尺寸和均匀分布，从而诱导增强的抗菌活性。与TSD相比改性的中孔二氧化硅涂覆的单壁碳纳米管（单壁碳纳米管@ MSIO ₂ -TSD）和商业的Ag纳米粒子，SWCNT的@ MSIO这种组合纳米系统₂ -TSD @银显示出对多药耐药性细菌更强的抗菌性能大肠埃希氏大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）通过破坏细菌细胞膜和快速释放银离子而在体外产生。此外，体内大鼠皮肤感染模型验证了SWCNTs @ mSiO ₂-TSD @ Ag具有显着提高的细菌清除能力，促进伤口愈合以及出色的生物相容性。因此，这种新颖的纳米平台显示了作为治疗临床耐药性感染的安全而有力工具的潜在潜力。