Abstract Fatty acids (FA) are minor compounds in beer and its raw materials, but can develop important roles in brewing and require monitoring. The aim of this work was to show the potential of capillary electrophoresis for fast hop analysis and to propose a simple method for the determination of saturated and unsaturated FA in different hop varieties. Sample preparation was investigated and evaluated and validation procedures were carried out to determine palmitic, stearic, oleic and linolenic FA in hops. Low values of relative standard deviation for areas (< 5.32%) and good recoveries (85-110%) were achieved. Laborious extraction and derivatization reactions were not necessary and only a saponification reaction was employed in sample preparation. Also, specific columns were not required and analysis time was only 10 min. The proposed methodology appears promising for faster monitoring of FA in terms of hop quality control in the brewing industry.

中文翻译：

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测定啤酒花中饱和和不饱和脂肪酸的 CZE-UV 方法

摘要 脂肪酸 (FA) 是啤酒及其原料中的次要化合物，但在酿造过程中可发挥重要作用并需要对其进行监测。这项工作的目的是展示毛细管电泳在快速酒花分析中的潜力，并提出一种测定不同酒花品种中饱和和不饱和 FA 的简单方法。对样品制备进行了调查和评估，并实施了验证程序，以确定啤酒花中的棕榈酸、硬脂酸、油酸和亚麻酸 FA。获得了较低的面积相对标准偏差值 (< 5.32%) 和良好的回收率 (85-110%)。不需要费力的提取和衍生反应，样品制备中仅使用皂化反应。此外，不需要特定的色谱柱，分析时间仅为 10 分钟。