Abstract The present study examined whether hop proanthocyanidins (PACs) or polyphenols could effectively protect alcohol-induced toxicity in human neuroblastoma SH-SY5Y cells. An in-vitro experiment indicated that alcohol could damage the SH-SY5Y cells. The degree of damage was correlated with the alcohol concentration. Cell damage was measured by cell viability, using the MTT assay, and cell apoptosis (programmed cell death) using the TUNEL assay. The results showed that 4 g/L alcohol in-vitro reduced cell viability to 71.7% and caused 34.7% cell apoptosis. Using this model, pre-treatments with 50-75 mg/L hop PACs retained 91% cell viability and inhibited cell apoptosis to below 15%. In addition, it was speculated that the mechanism underlying this neuroprotective effect of PACs might be closely associated with intracellular reactive oxygen species (ROS) levels. This in-vitro experiment demonstrated that the addition of 50-75 mg/L of PACs protected the nerve cells from the damage caused by 4 g/L of alcohol. To date, the impacts of PACs in the brains of humans are not known, however, if blood alcohol concentrations reach 4-5 g/L, severe central nervous system depression, coma, and possibly death can result. Responses of the SH-SY5Y cells might lend an indication of potential neuroprotective effects of hop PACs in alcohol-induced neurotoxicity.

中文翻译：

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啤酒花原花青素对酒精诱导的 SH-SY5Y 细胞损伤的保护作用

摘要 本研究探讨了啤酒花原花青素 (PAC) 或多酚是否能有效保护人神经母细胞瘤 SH-SY5Y 细胞中酒精诱导的毒性。体外实验表明，酒精会损伤 SH-SY5Y 细胞。损伤程度与酒精浓度相关。使用 MTT 测定法通过细胞活力测量细胞损伤，并使用 TUNEL 测定法通过细胞凋亡（程序性细胞死亡）来测量。结果表明，4 g/L 的体外酒精将细胞活力降低至 71.7%，并导致 34.7% 的细胞凋亡。使用该模型，用 50-75 mg/L 啤酒花 PAC 预处理保留 91% 的细胞活力并将细胞凋亡抑制到 15% 以下。此外，据推测，PAC 的这种神经保护作用的机制可能与细胞内活性氧 (ROS) 水平密切相关。该体外实验表明，添加 50-75 mg/L 的 PAC 可以保护神经细胞免受 4 g/L 酒精造成的损伤。迄今为止，PAC 对人类大脑的影响尚不清楚，但是，如果血液酒精浓度达到 4-5 克/升，可能会导致严重的中枢神经系统抑制、昏迷，甚至可能导致死亡。SH-SY5Y 细胞的反应可能表明啤酒花 PAC 在酒精诱导的神经毒性中具有潜在的神经保护作用。PAC 对人类大脑的影响尚不清楚，但是，如果血液酒精浓度达到 4-5 克/升，可能会导致严重的中枢神经系统抑制、昏迷，甚至可能导致死亡。SH-SY5Y 细胞的反应可能表明啤酒花 PAC 在酒精诱导的神经毒性中具有潜在的神经保护作用。PAC 对人类大脑的影响尚不清楚，但是，如果血液酒精浓度达到 4-5 克/升，可能会导致严重的中枢神经系统抑制、昏迷，甚至可能导致死亡。SH-SY5Y 细胞的反应可能表明啤酒花 PAC 在酒精诱导的神经毒性中具有潜在的神经保护作用。