Innovative design of a methodology for the simultaneous determination of compounds by kinetic-spectroscopy three-dimensional chemiluminescence.,Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

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Innovative design of a methodology for the simultaneous determination of compounds by kinetic-spectroscopy three-dimensional chemiluminescence.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.saa.2020.118045
José Antonio Murillo Pulgarín ₁ , Luisa F García Bermejo ₁ , M Nieves Sánchez García ₁ , Ignacio Sánchez-Ferrer Robles ₁

Affiliation

This report presents a novel methodology based in the quickly acquisition of full UV-vis range spectra combined with the joint determination of kinetic parameters and chemiluminescence signals. Then this technique allows obtaining three-dimensional chemiluminescence spectra profiles containing kinetic and spectroscopic information. That is especially useful for resolving mixtures of luminophors because of more analytical information for each analyte is available increasing the selectivity relative to conventional chemiluminescence methods. To accomplish this, a conventional Back-Thinned CCD detector is used and the three-dimensional chemiluminescence spectra is subsequently processed so that two-dimensional spectra with different trajectories can be obtained by dedicated software CLTotal. The potential of the proposed analytical methodology was assessed for the simultaneous determination of two polycyclic aromatic hydrocarbons. The chemiluminescent reaction between DNPO and hydrogen peroxide induced by their presence was used to record 3D spectra and the software CLTotal to subsequently construct linear variable-angle trajectories in the spectra, in order to obtain more selective 2D spectra facilitating the simultaneous determination of the analytes. The results of the statistical analysis testify to the usefulness of the proposed method for the intended purpose.

中文翻译：

通过动力学光谱三维化学发光同时测定化合物的方法的创新设计。

本报告提出了一种新颖的方法，该方法基于快速获取完整的UV-vis范围光谱以及联合确定动力学参数和化学发光信号的方法。然后，该技术可以获取包含动力学和光谱信息的三维化学发光光谱图。这对于解析发光体的混合物特别有用，因为可以获得每种分析物的更多分析信息，从而相对于常规化学发光方法提高了选择性。为了实现这一点，使用了传统的背照式CCD检测器，随后对三维化学发光光谱进行了处理，从而可以通过专用软件CLTotal获得具有不同轨迹的二维光谱。评价了拟议的分析方法的潜力，可同时测定两种多环芳烃。DNPO和过氧化氢的存在引起的化学发光反应用于记录3D光谱，软件CLTotal用于随后在光谱中构建线性可变角度轨迹，以便获得更具选择性的2D光谱，从而有利于同时测定分析物。统计分析的结果证明了所提出的方法对于预期目的的有用性。DNPO和过氧化氢的存在引起的化学发光反应用于记录3D光谱，软件CLTotal用于随后在光谱中构建线性可变角度轨迹，以便获得更具选择性的2D光谱，从而有利于同时测定分析物。统计分析的结果证明了所提出的方法对于预期目的的有用性。DNPO和过氧化氢的存在引起的化学发光反应用于记录3D光谱，软件CLTotal用于随后在光谱中构建线性可变角度轨迹，以便获得更具选择性的2D光谱，从而有利于同时测定分析物。统计分析的结果证明了所提出的方法对于预期目的的有用性。

更新日期：2020-01-08

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