First report and genetic diversity of porcine bufavirus in China.,Virology Journal

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First report and genetic diversity of porcine bufavirus in China.
Virology Journal ( IF 4.0 ) Pub Date : 2020-01-06 , DOI: 10.1186/s12985-019-1278-6
Yan-Kuo Sun _{1,

2} , Yong-Jie Chen _{1,

2} , Yu Cai _{1,

2} , Di-Hua Zhu _{1,

2} , Hao-Ming Pan _{1,

2} , Ying-Fang Wei _{1,

2} , Xiao-Liang Han _{1,

2} , Chi-Hai Ji _{1,

2} , Gang Lu _{1,

2} , Heng Wang _{1,

2} , Chun-Quan Ma ₃ , Gui-Hong Zhang _{1,

2}

Affiliation

BACKGROUND Bufavirus is a newly discovered zoonotic virus reported in numerous mammals and humans. However, the epidemiological and genetic characteristics of porcine bufaviruses (PBuVs) in China remain unclear. METHODS To detect PBuVs in China, 384 samples (92 fecal and 292 serum specimens) were collected from 2017 to 2018, covering six provinces in China, and were evaluated by nested PCR. Further, the positive samples from different provinces were selected to obtain the complete genome of Chinese PBuVs. RESULTS The prevalence rate of PBuV was 16.7% in Chinese domestic pigs in the Guangdong, Guangxi, Fujian, Jiangxi, Anhui, and Henan provinces. Additionally, the positive rate of fecal specimens was higher than that of the serum samples. Next, we sequenced nine near-complete genomes of Chinese field PBuV strains from different provinces. Homology and phylogenetic analyses indicated that Chinese PBuVs have high genetic variation (93.3-99.2%), showed higher nucleotide identity with an Austrian PBuV strain (KU867071.1), and developed into different branches within the same cluster. CONCLUSION To our knowledge, this is the first report on PBuV in China, expanding the geographic boundaries of PBuV circulation. Our data demonstrate that PBuVs are widely distributed in the six Chinese provinces. Moreover, these Chinese PBuVs exhibit genetic variation and continuous evolution characteristics. Taken together, our findings provide a foundation for future studies on bufaviruses.

更新日期：2020-04-22

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