BACKGROUND Cases of colonization or infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) are frequently reported in people who work with animals, including veterinary personnel. The aim of this study was to determine the prevalence of MRSA colonization among veterinary professionals. A total of 134 nasal swabs from healthy attendees of a veterinary conference held in the Czech Republic were tested for presence of MRSA. The stains were further genotypically and phenotypically characterized. RESULTS Nine isolated MRSA strains were characterized with sequence type (ST), spa type (t) and Staphylococcal Cassette Chromosome mec type. Five different genotypes were described, including ST398-t011-IV (n = 5), ST398-t2330-IV (n = 1), ST398-t034-V (n = 1), ST225-t003-II (n = 1) and ST4894-t011-IV (n = 1). The carriage of the animal MRSA strain was confirmed in 8 cases, characteristics of one strain corresponded to the possible nosocomial origin. Among animal strains were described three spa types (t011, t034, t2330) belonging into one dominating clonal complex spa-CC11. CONCLUSION According to our results, the prevalence of nasal carriage of MRSA in veterinary personnel is 6.72%. Although we described an increase compared to the results of previous study (year 2008), the prevalence in the Czech Republic is still remaining lower than reported from neighboring countries. Our results also indicate that healthcare - associated MRSA strains are still not spread among animals.

中文翻译：

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2017年捷克共和国兽医专业人士的耐甲氧西林金黄色葡萄球菌。

背景技术由耐甲氧西林的金黄色葡萄球菌（MRSA）引起的定植或感染病例经常在与动物打交道的人中包括兽医人员中报道。这项研究的目的是确定兽医专业人员中MRSA定植的普遍程度。在捷克共和国举行的一次兽医会议的健康参与者中，总共对134个鼻拭子进行了MRSA检测。进一步在基因型和表型上表征了染色。结果对9株分离的MRSA菌株进行了序列类型（ST），spa类型（t）和葡萄球菌盒式染色体mec类型鉴定。描述了五种不同的基因型，包括ST398-t011-IV（n = 5），ST398-t2330-IV（n = 1），ST398-t034-V（n = 1），ST225-t003-II（n = 1）和ST4894-t011-IV（n = 1）。在8例病例中证实了动物MRSA菌株的携带，一种菌株的特征与可能的医院起源相对应。在动物品系中，描述了三种spa类型（t011，t034，t2330），它们属于一种主要的克隆复合体spa-CC11。结论根据我们的研究结果，兽医人员经鼻携带MRSA的患病率为6.72％。尽管我们描述了与以前的研究结果（2008年）相比有所增加，但捷克共和国的患病率仍然低于邻国的报告。我们的结果还表明，与医疗相关的MRSA菌株仍未在动物中传播。t2330）属于一个主要的克隆复合体spa-CC11。结论根据我们的研究结果，兽医人员经鼻携带MRSA的患病率为6.72％。尽管我们描述了与以前的研究结果（2008年）相比有所增加，但捷克共和国的患病率仍然低于邻国的报告。我们的结果还表明，与医疗保健相关的MRSA菌株仍未在动物中传播。t2330）属于一个主要的克隆复合体spa-CC11。结论根据我们的研究结果，兽医人员经鼻携带MRSA的患病率为6.72％。尽管我们描述了与以前的研究结果（2008年）相比有所增加，但捷克共和国的患病率仍然低于邻国的报告。我们的结果还表明，与医疗相关的MRSA菌株仍未在动物中传播。