BACKGROUND Efficient organogenesis induction in eggplant (Solanum melongena L.) is required for multiple in vitro culture applications. In this work, we aimed at developing a universal protocol for efficient in vitro regeneration of eggplant mainly based on the use of zeatin riboside (ZR). We evaluated the effect of seven combinations of ZR with indoleacetic acid (IAA) for organogenic regeneration in five genetically diverse S. melongena and one S. insanum L. accessions using two photoperiod conditions. In addition, the effect of six different concentrations of indolebutyric acid (IBA) in order to promote rooting was assessed to facilitate subsequent acclimatization of plants. The ploidy level of regenerated plants was studied. RESULTS In a first experiment with accessions MEL1 and MEL3, significant (p < 0.05) differences were observed for the four factors evaluated for organogenesis from cotyledon, hypocotyl and leaf explants, with the best results obtained (9 and 11 shoots for MEL1 and MEL3, respectively) using cotyledon tissue, 16 h light / 8 h dark photoperiod conditions, and medium E6 (2 mg/L of ZR and 0 mg/L of IAA). The best combination of conditions was tested in the other four accessions and confirmed its high regeneration efficiency per explant when using both cotyledon and hypocotyl tissues. The best rooting media was R2 (1 mg/L IBA). The analysis of ploidy level revealed that between 25 and 50% of the regenerated plantlets were tetraploid. CONCLUSIONS An efficient protocol for organogenesis of both cultivated and wild accessions of eggplant, based on the use of ZR, is proposed. The universal protocol developed may be useful for fostering in vitro culture applications in eggplant requiring regeneration of plants and, in addition, allows developing tetraploid plants without the need of antimitotic chemicals.

中文翻译：

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基于玉米蛋白核糖苷的高效器官发生方案，用于茄子的体外再生。

背景技术茄子（Solanum melongena L.）中有效的器官发生诱导对于多种体外培养应用是必需的。在这项工作中，我们旨在开发一种主要基于玉米素核糖苷（ZR）的茄子有效体外再生的通用方案。我们使用两种光周期条件，评估了ZR与吲哚乙酸（IAA）的七种组合对五种遗传多样的S. melongena和一种S. insanum L.品种的器官发生再生的影响。另外，评估了六种不同浓度的吲哚丁酸（IBA）促进生根的作用，以促进植物的随后适应。研究了再生植物的倍性水平。结果在首次登录MEL1和MEL3的实验中，显着（p <0。05）在子叶组织，16 h光照/ 8 h黑暗光照条件下，从子叶，下胚轴和叶外植体的器官发生的四个评估因素中观察到差异，获得最佳结果（MEL1和MEL3分别为9和11枝）。 ，以及中度E6（2 mg / L的ZR和0 mg / L的IAA）。在其他四个种质中测试了最佳条件组合，并确认了同时使用子叶和下胚轴组织时，每个植株的再生效率高。最好的生根培养基是R2（1 mg / L IBA）。倍性水平的分析表明，再生小植株中有25％至50％是四倍体。结论基于ZR的使用，提出了一种栽培茄子和野生茄子的器官发生的有效方案。